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Pathological diagnosis kit, and rapid processing technology for tissue slices

A technology for pathological diagnosis and tissue slices, which is applied in the preparation of test samples, etc., can solve the problems of short processing time, small space occupation, and large space occupation of tissue slices for pathological diagnosis, and achieves the benefits of medical services, portability, The effect of improving utilization

Active Publication Date: 2017-12-19
YANGZHOU NO 1 PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0061] The purpose of the present invention is to provide a kit for pathological diagnosis and a rapid processing technology for tissue slices, so as to solve the problems of long time consumption, large consumption of reagents, large space occupation and environmental pollution in the process of fixation, dehydration and staining of existing tissue slices ;It has the characteristics of small space occupation, easy to carry, short processing time for tissue slices for pathological diagnosis, and can be used for different types of tissue slices for pathological diagnosis.

Method used

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  • Pathological diagnosis kit, and rapid processing technology for tissue slices
  • Pathological diagnosis kit, and rapid processing technology for tissue slices
  • Pathological diagnosis kit, and rapid processing technology for tissue slices

Examples

Experimental program
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Effect test

Embodiment 1

[0113] A pathological diagnosis kit for pathological diagnosis, containing four wash bottles with identification marks, which are marked as No. 1 bottle, No. 2 bottle, No. 3 bottle and No. 4 bottle.

[0114] Bottle No. 1: contains 85ml of Harri hematoxylin stain and 42ml of Clarke's fixative;

[0115] In bottle 1, Harri hematoxylin dye solution contains the following components by weight: 1 part of hematoxylin, 7.5 parts of absolute ethanol, 19 parts of potassium aluminum sulfate, 190 parts of distilled water, 0.55 parts of mercury oxide, 6 parts of glacial acetic acid When preparing, use absolute ethanol to dissolve hematoxylin, heat with distilled water to dissolve potassium aluminum sulfate, then combine the two liquids to boil, add mercury oxide, continue to heat and stir the solution to a deep purple, then cool with ice water to recover After reaching room temperature, filter for use, then add glacial acetic acid, mix and filter to obtain Harri hematoxylin dye solution;

[0116...

Embodiment 2

[0129] A pathological diagnosis kit for pathological diagnosis, containing four wash bottles with identification marks, which are marked as No. 1 bottle, No. 2 bottle, No. 3 bottle and No. 4 bottle.

[0130] Bottle No. 1: contains 95ml of Harri hematoxylin staining solution and 38ml of Clarke's fixative;

[0131] In bottle 1, Harri hematoxylin dye solution contains the following components by weight: 1 part of hematoxylin, 8.5 parts of absolute ethanol, 20 parts of potassium aluminum sulfate, 200 parts of distilled water, 0.5 parts of mercury oxide, ice 8 parts of acetic acid; when preparing, use absolute ethanol to dissolve hematoxylin, heat with distilled water to dissolve potassium aluminum sulfate, then combine the two liquids and boil, add mercury oxide, continue to heat and stir the solution to dark purple, then cool with ice water After returning to room temperature, filter for use, then add glacial acetic acid, mix and filter to obtain Harri hematoxylin dye solution;

[0132...

Embodiment 3

[0141] A pathological diagnosis kit for pathological diagnosis, containing four wash bottles with identification marks, which are marked as No. 1 bottle, No. 2 bottle, No. 3 bottle and No. 4 bottle.

[0142] Bottle No. 1: contains 90ml of Harri hematoxylin stain and 40ml of Clarke's fixative;

[0143] In bottle 1, Harri hematoxylin dye solution contains the following components by weight: 1 part of hematoxylin, 8 parts of absolute ethanol, 21 parts of potassium aluminum sulfate, 210 parts of distilled water, 0.45 parts of mercury oxide, 6.5 parts of glacial acetic acid When preparing, use absolute ethanol to dissolve hematoxylin, heat with distilled water to dissolve potassium aluminum sulfate, then combine the two liquids to boil, add mercury oxide, continue to heat and stir the solution to a deep purple, then cool with ice water to recover After reaching room temperature, filter for use, then add glacial acetic acid, mix and filter to obtain Harri hematoxylin dye solution;

[0144...

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Abstract

The invention relates to a pathological diagnosis kit, and a use method thereof. The kit comprises four washing bottles having identification marks, wherein the first bottle is filled with a Harri hematoxylin dye liquor and a Clarke fluid, the second bottle is filled with alcohol-soluble Eosin Y, anhydrous ethanol, distilled water and lithium carbonate, the third bottle is filled with a clarifier, and the fourth bottle is filled with a neutral quick-drying glue used for wet mounting. The kit greatly shortens the processing time of existing tissue slices, reduces the processing process link and the use amount of reagents, facilitates the development of medical service in remote regions, realizes field inspection during gratuitous treatment and general survey in the remote regions, allows the pathological diagnosis result to be seen in the field, and facilitates the improvement of the medical service level in the remote regions.

Description

Technical field [0001] The invention relates to a kit for pathological diagnosis and a method of use thereof, including fixation, dehydration and staining of tissue pieces. Background technique [0002] In the prior art, there is a kit for tumor pathological diagnosis and a method for staining tissue sections. Its application number: 201310152840.5, application date: 2013-04-27, publication number: CN103245789A, publication date: 2013-08 -14. The kit for pathological diagnosis of tumors includes probes for pathological diagnosis of tumors; the probes include gold nanoclusters, antibodies to tumor-specific markers, and Coomassie brilliant blue, and the gold nanoclusters are compatible with The antibody of the tumor-specific marker is connected by click chemistry, and the Coomassie Brilliant Blue is electrostatically bound to the gold nanocluster of the antibody of the tumor-specific marker; wherein, the gold nanocluster The molar ratio of the antibody of the tumor-specific marker...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/30G01N1/34G01N1/42G01N1/36
Inventor 王磊戚庭月颜云
Owner YANGZHOU NO 1 PEOPLES HOSPITAL
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