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Anti-aging face filler based on umbilical cord mesenchymal stem cells and preparation method of anti-aging face filler

A stem cell, anti-aging technology, applied in the field of anti-aging facial fillers for beauty, can solve the problems of no skin anti-aging repair beauty products, stem cell characteristic damage, false signals, etc., to promote hematopoietic recovery function and effect maintenance time Long, great results

Inactive Publication Date: 2017-12-22
HENAN YINFENG BIOENG CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Increased survival time and number of replications lead to accumulation of faulty signals at the genetic level within stem cells, meaning that even when these cells can be obtained and expanded, their stem cell properties are compromised
[0005] At present, there is no beauty product for skin anti-aging repair using umbilical cord mesenchymal stem cells

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] An anti-aging facial filler based on umbilical cord mesenchymal stem cells, composed of hyaluronic acid, umbilical cord mesenchymal stem cells and platelet-rich plasma.

[0022] The content of umbilical cord mesenchymal stem cells is 0.5×10 6 ~1×10 6 pieces / ml.

[0023] The volume ratio of hyaluronic acid to platelet-rich plasma is 1:1.

[0024] The volume fractions of the hyaluronic acid and the platelet-rich plasma are both 50%.

[0025] A preparation method of an anti-aging facial filler based on umbilical cord mesenchymal stem cells, comprising the following steps:

[0026] (1) Prepare umbilical cord mesenchymal stem cells; the specific method is:

[0027] Take umbilical cord blood, mix with an equal amount of PBS buffer, slowly drop into an equal amount of lymphocyte separation medium, and centrifuge at 650g for 15 minutes; extract buffy coat cells, wash and centrifuge with an equal amount of PBS buffer, and base culture medium ( DMEM / F12, 10% fetal bovine ser...

Embodiment 2

[0031] An anti-aging facial filler based on umbilical cord mesenchymal stem cells, composed of hyaluronic acid, umbilical cord mesenchymal stem cells and platelet-rich plasma.

[0032] The content of umbilical cord mesenchymal stem cells was 1×10 6 ~2×10 6 pieces / ml.

[0033] The volume ratio of hyaluronic acid to platelet-rich plasma is 1:2.

[0034] The volume fractions of the hyaluronic acid and the platelet-rich plasma are both 30%.

[0035] A preparation method of an anti-aging facial filler based on umbilical cord mesenchymal stem cells, comprising the following steps:

[0036] (1) Prepare umbilical cord mesenchymal stem cells; the specific method is:

[0037] Take umbilical cord blood, mix with an equal amount of PBS buffer, slowly drop into an equal amount of lymphocyte separation medium, and centrifuge at 650g for 15 minutes; extract buffy coat cells, wash and centrifuge with an equal amount of PBS buffer, and base culture medium ( DMEM / F12, 10% fetal bovine seru...

Embodiment 3

[0041] An anti-aging facial filler based on umbilical cord mesenchymal stem cells, composed of hyaluronic acid, umbilical cord mesenchymal stem cells and platelet-rich plasma.

[0042] The content of umbilical cord mesenchymal stem cells is 3×10 6 ~4×10 6 pieces / ml.

[0043] The volume ratio of hyaluronic acid to platelet-rich plasma is 1:3.

[0044] The volume fractions of the hyaluronic acid and the platelet-rich plasma are both 40%.

[0045] A preparation method of an anti-aging facial filler based on umbilical cord mesenchymal stem cells, comprising the following steps:

[0046] (1) Prepare umbilical cord mesenchymal stem cells; the specific method is:

[0047] Take umbilical cord blood, mix with an equal amount of PBS buffer, slowly drop into an equal amount of lymphocyte separation medium, and centrifuge at 650g for 15 minutes; extract buffy coat cells, wash and centrifuge with an equal amount of PBS buffer, and base culture medium ( DMEM / F12, 10% fetal bovine serum...

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Abstract

The invention discloses an anti-aging face filler based on umbilical cord mesenchymal stem cells and a preparation method of the anti-aging face filler. The anti-aging face filler is composed of hyaluronic acid, the umbilical cord mesenchymal stem cells and platelet-rich plasma, wherein the content of the umbilical cord mesenchymal stem cells is 0.5x10<6>-5x10<6> / ml; the volume ratio of hyaluronic acid to the platelet-rich plasma is 1: (1-3); the umbilical cord mesenchymal stem cells have the strong efficacies of regulating immunity, promoting hematopoiesis recovery, and repairing injured or lesion tissues and organs. For the anti-aging face filler based on the umbilical cord mesenchymal stem cells, the umbilical cord mesenchymal stem cells are adopted for carrying out anti-aging repair on the skin, the effect is excellent after use of the anti-aging face filler, the filled part is natural and plump, and the effect maintaining time is long.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an anti-aging facial filler for cosmetic use using umbilical cord mesenchymal stem cells. Background technique [0002] Umbilical cord mesenchymal stem cells (Mesenchymal Stem Cells, MSCs) refer to a kind of multifunctional stem cells existing in the umbilical cord tissue of newborns, which can differentiate into many kinds of tissue cells and have broad clinical application prospects. [0003] Umbilical cord mesenchymal stem cells have high differentiation potential and can differentiate in multiple directions. It has broad clinical application prospects in tissue engineering of bone, cartilage, muscle, tendon, ligament, nerve, liver, endothelium and cardiac muscle. It has been reported that mesenchymal stem cells were isolated from human umbilical cord, and the cell content and proliferation ability were superior to those of bone marrow mesenchymal stem cells, the immu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/98C12N5/0775A61Q19/08
CPCA61K8/982A61Q19/08C12N5/0665C12N2509/00
Inventor 朱学义王小龙程志远陈乐陈艳普魏天迪
Owner HENAN YINFENG BIOENG CO LTD
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