Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Non-amplification nucleic acid hybrid capture system and application thereof in nucleic acid test

A nucleic acid hybridization and non-amplification technology, which is applied in the non-amplification nucleic acid hybridization capture detection system and its application in nucleic acid detection, can solve the problems of high detection cost, complicated operation, and inability to truly reflect nucleic acid. achieve low cost

Inactive Publication Date: 2017-12-29
NANJING UNIV
View PDF4 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Problems to be solved in the present invention: In view of the shortcomings of existing nucleic acid detection methods, such as relatively complicated operation, corresponding amplification and detection instruments are required, resulting in high detection costs, which cannot truly reflect the initial amount of nucleic acid

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Non-amplification nucleic acid hybrid capture system and application thereof in nucleic acid test
  • Non-amplification nucleic acid hybrid capture system and application thereof in nucleic acid test
  • Non-amplification nucleic acid hybrid capture system and application thereof in nucleic acid test

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0038] Specific examples are provided below to further illustrate the technical solution of the present invention, but the application of the technology of the present invention is not limited to the examples.

[0039] 1 Extraction of HPV16 RNA

[0040] Extract RNA from HPV16 positive or negative samples.

[0041] 2 Design combinations for HPV16 RNA oligonucleotide probes

[0042] According to the HPV16 genome sequence published by NCBI (reference sequence: NC_001526.2), the biotin-labeled oligonucleotide probes for each gene fragment of HPV16 are designed, and the sequence is as shown in Table 1:

[0043] Table 1 Probe sequence

[0044]

[0045] 3 Establishment of immunological detection method for HPV16 RNA

[0046] 3.1 Annealing hybridization

[0047] Anneal and hybridize the above probe system with HPV16 RNA template, hybridization system: 10 μL 10× annealing buffer, 13 μL or 12 μL probe mixture, 10 μL RNA template solution, supplemented with DEPC H 2 0 to 100 μL; ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of molecular biology, immunology and nucleic acid chemistry and in particular relates to a non-amplification nucleic acid hybrid capture system and application thereof. The kit is an immunoassay method for capturing hybrid nucleic acids based on a non-amplification anti-nucleic antibody; inverse transcription, PCR (Polymerase Chain Reaction) and other amplification technologies are not needed in the detection process, while a probe complementary to a to-be-detected nucleic acid is subjected to annealing hybridization with the to-be-detected nucleic acid so as to form a structure which can be recognized by the anti-nucleic antibody, and the detection signal is amplified by utilizing enzyme linked immunosorbent assay. The detection method has the advantages of being rapid, low in cost, high in sensitivity, high in specificity, free in complicated amplification and detection instruments and capable of detecting initial viral load.

Description

technical field [0001] The invention belongs to the technical fields of molecular biology, immunology and nucleic acid chemistry. In particular, it relates to a non-amplified nucleic acid hybrid capture detection system and its application in nucleic acid detection. Background technique [0002] Nucleic acid is a biological macromolecular compound synthesized by many nucleotides, and is one of the most basic substances of life. Nucleic acid widely exists in all animal and plant cells and microorganisms. Different nucleic acids have different chemical compositions and nucleotide arrangement sequences. According to different chemical composition, nucleic acid can be divided into ribonucleic acid (referred to as RNA) and deoxyribonucleic acid (referred to as DNA). [0003] Currently, nucleic acid detection methods are mainly divided into DNA detection and RNA detection. For DNA detection, there are methods based on in situ hybridization and DNA sequencing, based on branched...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/70
CPCC12Q1/6804C12Q1/708C12Q2565/518C12Q2565/519C12Q2527/125
Inventor 沈萍萍丁森黄亚红刘培
Owner NANJING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com