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Enhanced cell microcarrier perfusion culture entrapment method

A technology of cell microcarrier and perfusion culture, which is applied in the interception field of cell microcarrier perfusion culture, can solve the problems of low solid-liquid separation efficiency, loss of microcarriers, and difficult control of liquid level, etc., so as to improve perfusion volume and retention efficiency, cell The effect of little damage

Active Publication Date: 2018-01-05
SHANDONG YIDU BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the volume of perfusion required per day is large, it is difficult to meet the requirements with a settling column, which will cause the loss of microcarriers, low solid-liquid separation efficiency, and the liquid level is not easy to control

Method used

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  • Enhanced cell microcarrier perfusion culture entrapment method

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Embodiment Construction

[0019] The present invention will be further described below in conjunction with the accompanying drawings.

[0020] Enhanced interception device and method for cell microcarrier perfusion culture, the device includes a settling column body 2, a settling chamber 4, a layered funnel 3, a medium-microcarrier inlet 6, a medium outlet 1, a microcarrier return port 5, Observation window 7, settling column 8, bioreactor 9, peristaltic pump 10, a layered funnel 3 is provided inside the device body, the bottom of the layered funnel 3 is a settling chamber 4, and a medium-microcarrier is opened on the settling chamber 4 Inlet 6, the bottom of the settling chamber 4 is connected to the connecting pipe, the bottom of the connecting pipe is the medium-microcarrier return port 5, the top of the layered funnel 3 is the medium outlet 1, and there is an observation window at the layered funnel 3 on one side of the device body 7.

[0021] Both the medium-microcarrier inlet 6 and the microcarr...

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Abstract

The invention provides an enhanced cell microcarrier perfusion culture entrapment device and method. A device body is internally provided with a layered funnel, a sedimentation chamber is formed in the bottom of the layered funnel, a culture medium-microcarrier inlet is formed in the sedimentation chamber, a connecting pipe is connected to the bottom of the sedimentation chamber, a culture medium-microcarrier reflux inlet is formed in the lower portion of the connecting pipe, a culture inlet is formed in the top of the layered funnel, and an observation window is formed in the portion, at oneside of the device body, of the layered funnel. The device has the advantages that the functions of solid-liquid sedimentation separation and liquid level control are achieved, cell microcarrier perfusion volume and entrapment efficiency are increased, the problem that a microcarrier is not prone to sedimentation so as to run off due to the fact that the liquid inlet velocity is too high is solved, and damage to cell is small in the microcarrier entrapment process.

Description

technical field [0001] The invention relates to the field of perfusion culture of cell microcarriers, in particular to an enhanced retaining method for perfusion culture of cell microcarriers. Background technique [0002] Using a perfusion culture system instead of the traditional batch or batch-supplement production process can greatly increase the cell density and the yield of the target product, and has been increasingly used in the biopharmaceutical industry. For efficient perfusion culture, entrapment of microcarriers is required. The interception mode of microcarriers can be divided into internal interception and external interception. Internal interception mainly includes technologies such as hollow fiber filtration and rotary filtration, and external interception methods include external filtration, centrifugation, and sedimentation. However, in practical applications, these devices have limited interception efficiency, high cost, or short service life and Can not...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/36C12N5/00
Inventor 庄长鹰
Owner SHANDONG YIDU BIOTECH
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