Edible bacterial strain as well as preparation method and application thereof
A food-derived, strain-based technology, applied in the direction of biochemical equipment and methods, applications, and methods based on microorganisms, can solve problems such as potential safety hazards, achieve the effects of reducing miscellaneous gas and irritation, coordinating chemical components, and clean aftertaste
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Embodiment 1
[0031] 1. Isolation of Lactobacillus plantarum L14
[0032] 1. Medium
[0033] (1) Improved MRS solid medium
[0034] The improved MRS solid medium used in the present invention: peptone 10g / L, beef powder 8g / L, yeast powder 4g / L, glucose 20g / L, Tween-80 1g / L, K 2 HPO 4 2g / L, NaAc 5g / L, MgSO 4 0.2g / L, MnSO 4 0.05g / L, triammonium citrate 2g / L, CaCO 3 10g / L, bromocresol purple 0.04g / L, agar 15g / L, sterilized at 115°C for 20min;
[0035] (2) MRS liquid medium
[0036] The MRS liquid medium used in the present invention:
[0037] Peptone 10g / L, beef powder 8g / L, yeast powder 4g / L, glucose 20g / L, Tween-80 1g / L, K 2 HPO 4 2g / L, NaAc 5g / L, MgSO 4 0.2g / L, MnSO 4 0.05g / L, triammonium citrate 2g / L, sterilized at 115°C for 20min;
[0038] (3) Tobacco leaf culture medium
[0039]Grind tobacco leaves of variety K326 with 1-year aging into powder, weigh 0.8-1.0g of powder and 1.5g of agar, adjust to 100mL with water, sterilize at 115°C for 20min, cool and pour onto a pla...
Embodiment 2
[0076] Application of Lactobacillus plantarum L14
[0077] Plantarum L14 activated in MRS liquid medium for 15 hours was inoculated in 100 mL of MRS liquid medium at 8‰ (v / v) inoculation amount, and cultured at 34°C for 13 hours. At 9500rpm, centrifuge for 10min to precipitate the bacteria, wash the bacteria once with sterile deionized water, and then use sterile deionized water to make a concentration of 10 10 CFU / mL bacterial suspension, this is Lactobacillus plantarum L14 fermentation inoculum.
[0078] Take out shredded tobacco from the commercialized Yunyan Ziyun, spray 6mL Lactobacillus plantarum L14 fermentation agent on 28g shredded tobacco with a moisture content of 10%, and ferment in a constant temperature and humidity incubator (28°C, 60% humidity) for 14 days , made into cigarettes according to conventional methods, and used for sensory evaluation and smoking. For the control sample, an equal amount of sterile water was used instead of the fermentation agent, an...
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