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Blood cfDNA (cell free DNA) preservative, vacuum blood collection tube and preparation method of vacuum blood collection tube

A technology for vacuum blood collection tubes and preservatives, which is used in biochemical equipment and methods, medical science, and microbial determination/inspection, etc., which can solve the problem of inaccurate results, inability to effectively inhibit RNase enzyme activity, and inability to protect the stability of free DNA in plasma To prevent cracking and prolong storage time

Inactive Publication Date: 2018-01-12
北京恩吉思生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it cannot effectively inhibit the activity of RNase enzyme, so it cannot protect the stability of free DNA in plasma, resulting in inaccurate results of early tumor screening or fetal prenatal screening and diagnosis

Method used

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  • Blood cfDNA (cell free DNA) preservative, vacuum blood collection tube and preparation method of vacuum blood collection tube
  • Blood cfDNA (cell free DNA) preservative, vacuum blood collection tube and preparation method of vacuum blood collection tube

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The preparation of a blood cfDNA vacuum blood collection tube adopts the following steps:

[0022] (1) EDTA10mg, imidazole 20mg, glyceraldehyde 50mg, sodium chloride 1mg, guanidine hydrochloride 50mg and SDS 1mg;

[0023] (2) Dissolve each of the above-mentioned raw materials with 1000 mg of RNase-free purified water, and filter each dissolved component with a 0.22 μm membrane for subsequent use;

[0024] (3) mixing the above-mentioned prepared raw materials;

[0025] (4) Fill the above-mentioned mixed solution into a blood collection tube without additives, and pump it into a vacuum negative pressure, and the vacuum negative pressure is greater than 16kg / cm 2 , The maximum vacuum error is ±10%.

[0026] Ready-to-use cfDNA vacuum blood collection tubes, among which the additive-free blood collection tubes are provided by Jiangsu Kangjie Medical Instrument Co., Ltd.

Embodiment 2-6

[0028] Examples 2-6 are the preparation of blood cfDNA vacuum blood collection tubes. The difference from Example 1 is that the amounts of components used are different. See Table 1 for details.

[0029] Table 1 The proportioning ratio of each component

[0030]

Embodiment 7

[0032] The blood cfDNA vacuum blood collection tubes prepared by the above examples were tested with existing common blood collection tubes containing EDTA and lithium heparin (Jiangsu Kangjie Medical Instrument Co., Ltd.) to investigate the effect of blood collection tubes on free DNA in blood.

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Abstract

The invention discloses a blood cfDNA (cell free DNA) preservative and a vacuum blood collection tube constituted by the blood cfDNA preservative, wherein the preservative consists of the following components in parts by weight: 1-200 parts of EDTA, 1-500 parts of imidazole, 1-200 parts of glyceraldehyde, 1-1000 parts of sodium chloride, 1-500 parts of guanidine hydrochloride, 1-1000 parts of SDSand 5000-7000 parts of purified water. The blood cfDNA vacuum blood collection tube is the vacuum blood collection tube containing the preservative. With the application of the blood cfDNA preservative provided by the invention, RNase enzyme activity can be effectively inhibited and free DNA in blood can be protected. The blood cfDNA vacuum blood collection tube is capable of inhibiting the RNaseenzyme activity and protecting the free DNA in the blood, and the vacuum blood collection tube is also capable of effectively immobilizing leukocyte and preventing the leukocyte from getting cracked,so that the circumstance that the free DNA is contaminated by the cracked leukocyte is prevented; the preservation duration of a blood sample at room temperature is effectively prolonged, and the vacuum blood collection tube is quite conducive to the long-distance transportation of a plasma sample; therefore, an effective preservation method is provided for collection, storage and transportation of tumor early screening samples or fetus prenatal screening and diagnosis samples.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a blood cfDNA preservation agent, a vacuum blood collection tube and a preparation method thereof. Background technique [0002] Cancer has become the leading cause of death in my country, accounting for a quarter of global cancer deaths. Compared with the United States, although its tumor incidence rate is 1.6 times (65% higher) than that in China, its 5-year survival rate is 1.8 times (81% higher) than in China. One of the main reasons for this difference is that the prevalence rate of early tumor screening and diagnosis in the United States is higher. Early tumor screening mainly adopts endoscopy, blood, imaging and other inspection methods, while for cfDNA (cell free DNA) in blood, That is, early screening of free DNA is a very important and convenient detection method. When a pregnant woman is pregnant, the DNA of the fetus will also be released into the mother's b...

Claims

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Application Information

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IPC IPC(8): C12Q1/6806A61B5/154
Inventor 王爱玲田春城
Owner 北京恩吉思生物科技有限公司
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