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Preparation method of cast-off cells in frozen section

A technology of frozen sections and exfoliated cells, which is applied in the preparation of test samples, etc., can solve the problems of complicated procedures, and achieve the effect of clear cells, wide application range, and good differential diagnosis

Active Publication Date: 2018-01-12
曾峰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, it is necessary to add saturated picric acid gelatin and centrifuge many times before the sediment can be obtained, and the procedure is too complicated

Method used

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  • Preparation method of cast-off cells in frozen section
  • Preparation method of cast-off cells in frozen section
  • Preparation method of cast-off cells in frozen section

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for preparing exfoliated cells in frozen sections, the specific steps are as follows:

[0031] a. Collect the pleural and ascites after the clinical examination of XXX 1;

[0032] b. Using a plastic test tube, take 100 ml of pleural and ascites fluid and centrifuge in a centrifuge at a speed of 2800 rpm for 10 minutes;

[0033] c. Discard the supernatant after taking out the plastic test tube, then cut off the upper part of the plastic test tube, leaving 2 cm of the upper part, and cut a crack on the edge of the plastic test tube with the length of the crack being 1.2 cm;

[0034] d. Use paraffin to make a wax model, and make a groove in the middle of the wax model for putting the plastic test tube with the upper part cut off, and the depth of the groove is 0.2 cm;

[0035] e. Put the plastic test tube with the upper part cut off into the groove of the wax mold, inject glue into the plastic test tube with the upper part cut off to cause overflow, and then cove...

Embodiment 2

[0039] A method for preparing exfoliated cells in frozen sections, the specific steps are as follows:

[0040] a. Collect the pleural and ascites after XX 2 clinical inspection and fixation;

[0041] b. Using a plastic test tube, take 25ml of pleural and ascites and put it into a centrifuge for centrifugation. The centrifugation rate is 3500 rpm and the centrifugation time is 5 minutes;

[0042] c. Discard the supernatant after taking out the plastic test tube, then cut off the upper part of the plastic test tube, leaving a 1.5cm portion, and cut a slit on the edge of the plastic test tube with a length of 1.4cm;

[0043] d. Use paraffin to make a wax model, and make a groove in the middle of the wax model for putting the plastic test tube with the upper part cut off, and the depth of the groove is 0.4cm;

[0044] e. Put the plastic test tube with the upper part cut off into the groove of the wax mold, inject glue into the plastic test tube with the upper part cut off to caus...

Embodiment 3

[0048] The conventional centrifugal smear and liquid-based film production methods currently used are as follows:

[0049] After centrifuging the test solution in the centrifuge, pour off the supernatant, leave a small amount of bottom liquid, put it into a disposable liquid-based cell collection bottle, put the collection bottle into the liquid-based cell preparation machine, and start the instrument to prepare After 6 minutes, open the protective cover of the instrument, take out the disposable glass slide coated with cells, fix, stain, seal the slide, and read the slide under the microscope. The result of the reading picture is as follows image 3 shown.

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Abstract

A preparation method of a cast-off cells in a frozen section specifically comprises the following steps: a, collecting hydrothorax and ascite which is clinically inspected and fixed; b, collecting hydrothorax and ascite through a plastic test tube, and then centrifuging through a centrifugal machine; c, removing the plastic test tube; removing the supernate; and shearing the upper part of the plastic test tube; d, preparing a die through a paraffin; e, arranging the plastic test tube of which the upper part is shorn into a concave die of the paraffin die; pouring glue into the test tube untilthe glue overflows from the test tube, and then covering a frozen section specimen holder; f, freezing the paraffin die which is covered with the frozen section specimen holder through a frozen section machine under the temperature of -25 DEG C; g, removing the plastic test tube after freezing; transferring on the frozen section machine; cutting into 5-7 microns sections; dyeing, and waiting for inspection. According to the preparation method, the prepared frozen section of the cast-off cells is viewed, and the viewing test shows that the cells are more than those of a general centrifugal smear and a liquid-based section, and the cells are concentrated, so that the sections can be treated as disease diagnosed basis with sufficient evidence, and good differentiation diagnosis is provided for the clinic. The method is simple, convenient, and wide in applicable scope.

Description

technical field [0001] The invention belongs to the technical field of medical detection methods, and in particular relates to a method for preparing exfoliated cells in frozen sections. Background technique [0002] For many years, the collection and diagnosis rate of exfoliated cells from pleural effusion is low, which is a difficult problem for pathologists. Although there are many new types of exfoliated cell collection equipment such as "liquid-based", most of them are not satisfactory. As early as more than 20 years ago, Chen Senlin and He Zhiding of Henan Provincial Cancer Hospital conducted research, discussion and application of exfoliated cells in frozen section technology. They put the submitted body fluid specimens into plastic test tubes, centrifuge to precipitate, discard the supernatant, add 0.5M PBS to wash, centrifuge several times, and then add 50-100ML 5% methylcellulose for later use. Then take 1.2 grams of agar and dissolve it in 20ML PBS solution to pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
Inventor 曾峰
Owner 曾峰