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Inducing method of bastard halibut allogynogenetic tetraploid

A technology of gynogenesis and tetraploidy, which is applied in the field of induction of gynogenesis and tetraploidy of flounder, can solve problems such as inability to fix

Active Publication Date: 2018-01-19
中国水产科学研究院北戴河中心实验站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The tetraploids obtained by this method have the genetic information of the parents, so it is impossible to fix some excellent traits that only the mother has.
For some female individuals with special traits, how to achieve tetraploidization cannot be solved by current technology

Method used

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  • Inducing method of bastard halibut allogynogenetic tetraploid
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  • Inducing method of bastard halibut allogynogenetic tetraploid

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Experimental program
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Effect test

Embodiment 1

[0049] 1) Collection of sperm and eggs

[0050] Fresh sea bream sperm and flounder eggs were collected from the Beidaihe Central Experimental Station of the Chinese Academy of Fishery Sciences.

[0051] 2) Sperm UV irradiation

[0052] Dilute the above sperm 40 times with diluent (Grüner's solution), and irradiate for 45 seconds under a 30cm ultraviolet lamp at a distance of 30w, and the irradiation dose is 70mJ / cm 2 .

[0053] 3) Artificial insemination:

[0054] The red sea bream sperm and flounder eggs irradiated by ultraviolet rays were mixed according to the number ratio of 1:10, and after stirring evenly, 10 times the volume of seawater at 15°C was added to it to obtain a mixture of sperm and eggs activated by seawater.

[0055] 4) Cold shock treatment inhibits the release of the second polar body:

[0056] After being activated in seawater for 3 minutes, the sperm-egg mixture was transferred to seawater at 0°C for cold shock treatment for 45 minutes, and then transf...

Embodiment 2

[0084] 1) Collection of sperm and eggs

[0085] Fresh sea bream sperm and flounder eggs were collected from the Beidaihe Central Experimental Station of the Chinese Academy of Fishery Sciences.

[0086] 2) Sperm UV irradiation

[0087] Dilute the above-mentioned sperm by 50 times with diluent (Grüner's solution), and irradiate for 60 seconds under a 32cm ultraviolet lamp at a distance of 32w, and the irradiation dose is 92mJ / cm 2 .

[0088] 3) Artificial insemination:

[0089] The red sea bream sperm and flounder eggs irradiated by ultraviolet rays are mixed according to the number ratio of 1-10, and after stirring evenly, 12 times the volume of seawater at 19° C. is added thereto to obtain the sperm-egg mixture activated by seawater.

[0090] 4) Cold shock treatment inhibits the release of the second polar body:

[0091] After being activated in seawater for 3 minutes, the sperm-egg mixture was transferred to seawater at 3°C ​​for cold shock treatment for 40 minutes, and ...

Embodiment 3

[0098] 1) Collection of sperm and eggs

[0099] Fresh sea bream sperm and flounder eggs were collected from the Beidaihe Central Experimental Station of the Chinese Academy of Fishery Sciences.

[0100] 2) Sperm UV irradiation

[0101] Dilute the above sperm 30 times with diluent (Grüner's solution), and irradiate for 30 seconds under a 28cm ultraviolet lamp at a distance of 28w, and the irradiation dose is 55mJ / cm 2 .

[0102] 3) Artificial insemination:

[0103] The red sea bream sperm and flounder eggs irradiated by ultraviolet rays were mixed according to the number ratio of 1:20, and after stirring evenly, 8 times the volume of seawater at 17°C was added to it to obtain the sperm-egg mixture activated by seawater.

[0104] 4) Cold shock treatment inhibits the release of the second polar body:

[0105] After being activated in seawater for 3 minutes, the sperm-egg mixture was transferred to seawater at 2°C for cold shock treatment for 50 minutes, and then transferred t...

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Abstract

The invention provides an inducing method of bastard halibut allogynogenetic tetraploid, and belongs to the technical field of a marine organism breeding technique. The inducing method includes stepsof performing ultraviolet irradiation on fresh red sea bream sperm to inactivate; mixing the inactivated red sea bream sperm with fresh bastard halibut ovum, mixing the obtained mixed fluid of sperm and ovum with seawater at 15-19 DEG C for 3 minutes; then transferring the mixed fluid of sperm and ovum to the seawater at 0-3 DEG C to perform cold shock treatment for 40-50 minutes; cultivating theprocessed mixed fluid of sperm and ovum for 45-71 minutes; selecting floating spawns from the mixed fluid of sperm and ovum and placing the spawns in an environment with pressure of 550-750 kg / cm2 toprocess for 5-7 minutes; performing the flowing hatching on the floating spawns after the static pressure treatment. The method applies the cold shock and static water pressure method to perform chromosome multiplying treatment on the ovum activated by inactivated heterologous sperm, and realizes the objective that the induced tetraploid only contains maternal inheritance information; meanwhile, the method has high inductivity.

Description

technical field [0001] The invention belongs to the technical field of marine biological breeding, and in particular relates to a method for inducing tetraploid gynogenetic flounder. Background technique [0002] Polyploidy breeding is an important technical means of fish breeding. In fish, triploids generally have the characteristics of fast growth and sterility, which can greatly increase the unit breeding output and increase income. The easiest way to mass-produce triploid seedlings required for commercial farming is through mating between tetraploid and diploid parents. Therefore, the preparation of tetraploid is of great significance in fish polyploid breeding. [0003] Although there are natural tetraploid fish in nature, the quantity is small and difficult to obtain, which cannot satisfy the application of fry. The traditional fish tetraploid induction is through the normal fertilization of sperm and eggs, and then inhibits cleavage to achieve chromosome doubling. ...

Claims

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Application Information

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IPC IPC(8): A01K61/10A01K61/17C12Q1/6888
CPCY02A40/81
Inventor 侯吉伦王玉芬王桂兴张晓彦孙朝徽都威赵雅贤于清海
Owner 中国水产科学研究院北戴河中心实验站
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