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Glioma prognostic marker circ6:4891713|4892379 and applications

A glioma and marker technology, which is applied in biochemical equipment and methods, microbial determination/examination, DNA/RNA fragments, etc., can solve the problems that the survival rate of glioma patients is not significantly improved, and the survival rate is not optimistic. , to achieve the effect of improving clinical treatment effect, good enrichment effect, and rapid and accurate grasp of the patient's condition

Inactive Publication Date: 2018-01-19
XIANGYA HOSPITAL CENT SOUTH UNIV
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Problems solved by technology

At present, although the diagnosis and treatment methods of glioma are in the stage of continuous improvement, the survival rate of glioma patients has not been significantly improved.
The diagnosis of glioma is still in the empirical stage based on clinical, pathological and imaging information, and once diagnosed, most of them are in the middle and advanced stages, and the survival rate after surgery is not optimistic

Method used

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  • Glioma prognostic marker circ6:4891713|4892379 and applications
  • Glioma prognostic marker circ6:4891713|4892379 and applications
  • Glioma prognostic marker circ6:4891713|4892379 and applications

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Embodiment Construction

[0019] The following embodiments are intended to further illustrate the present invention, rather than limit the present invention.

[0020] 1. Research object

[0021] 1. Six primary glioma cell lines were 1216, 1125, 1124C, 1124B, 1104, and 0128C, all provided by the Institute of Cancer, School of Basic Medicine, Central South University.

[0022] 2. The serum samples of 40 glioma patients were provided by Xiangya Hospital, and the 15 normal serum samples were healthy individuals who underwent community disease screening at the same time. The samples used for the research were collected at the same period, and the conditions of sampling, packaging and storage were the same.

[0023] 2. Research Methods

[0024] 1. Extraction of RNA from cells, exosomes secreted by cells, and glioma / normal serum exosomes

[0025] A. Extraction of cellular RNA

[0026] After the above six kinds of cells grow to an appropriate density, discard the medium, add 1ml Trizol reagent to the cultu...

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Abstract

The invention discloses a glioma prognostic marker Circ6:4891713|4892379 and applications. The fact that the exosome secreted by glioma cells has obvious accumulation on circ6:4891713|4892379 is firstly found; the expression level of circ6:4891713|4892379 in serum exosome of patients with glioma is found to be obviously increased compared with a normal control group (p=0.0005), ROC analysis showsthat circ6:4891713|4892379 has extremely high diagnosis value on glioma (AUC=0.906, p=0.001, and sensitivity and specificity are respectively 70.8% and 100%). Therefore, the early-phase and rapid non-invasively diagnosis on the patients with glioma can be made by detecting the expression level of circ6:4891713|4892379 in serum exosome of patients with glioma.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a serum circRNA marker Circ6:4891713|4892379 for glioma diagnosis, and the application of a reagent for detecting the marker for preparing glioma diagnostic preparations, and a kit. Background technique [0002] Glioma is the most frequent brain tumor in adults, accounting for 40.49% of intracranial tumors. From the time of diagnosis, the average life expectancy of patients with glioma is less than five years. In order to diagnose and treat glioma, a malignant disease highly correlated with genetic material, it is necessary to explore its pathogenesis at the level of molecular biology and from the expression of genetic information. At present, although the diagnosis and treatment methods of glioma are in the stage of continuous improvement, the survival rate of glioma patients has not improved significantly. The diagnosis of glioma is still in the empirical stage based on clinical, p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/113
Inventor 李沛瑶武明花孟舒娟范礼刘强王蓉
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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