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Aromatic compound detection method and kit

A technology of aromatic compounds and kits, applied in the field of analytical chemistry, to achieve the effects of improving detection sensitivity, wide application range, and improving ionization efficiency

Inactive Publication Date: 2020-06-12
APPLIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Taking the LC-MS detection method of estradiol as an example, it has been reported to use acid chlorides to derivatize estradiol, but this derivatization method is only effective for aromatic compounds with hydroxyl groups
However, no universal derivatization method for aromatic compounds that can improve the detection sensitivity of LC-MS has been reported yet.

Method used

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  • Aromatic compound detection method and kit
  • Aromatic compound detection method and kit
  • Aromatic compound detection method and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] The preparation of embodiment 1 diazonium salt

[0082] Weigh 31.5 mg of p-aminobenzenesulfonic acid, completely dissolve in 15 mL of NaOH solution with a mass fraction of 1%, add 12 mg of NaNO 2 , and then add 3mL of 1M HCl solution, the reaction generates diazonium benzenesulfonate.

Embodiment 2

[0083] The derivatization of embodiment 2 estradiol (Estradiol) and benzenesulfonate diazonium salt

[0084] S100, providing diazonium benzenesulfonate.

[0085] S200, using the provided diazonium benzenesulfonate to derivatize the aromatic compound estradiol, the concentration of estradiol is 5 μg / ml. The reaction equation is as follows:

[0086]

[0087] S300, testing the estradiol derivatives and underivatized estradiol produced by the reaction using an LC-MS detection method. see figure 2 , image 3 , Figure 4 and Figure 5 In the negative ion mode, the full-scan mass spectrogram is used to detect whether there is derivatized and underivatized estradiol in the sample to be tested, and then the single-ion monitoring ion chromatogram is used for quantitative testing. The diazo derivatization method can significantly improve the signal of estradiol single ion detection scanning quantitative test.

Embodiment 3

[0088] Embodiment 3 Diazo derivatization of estriol (Estriol) and benzenesulfonic acid diazonium salt

[0089] S100, providing diazonium benzenesulfonate.

[0090] S200, use the provided diazonium benzenesulfonate to derivatize the aromatic compound estriol, and the concentration of estriol is 5 μg / ml. The reaction equation is as follows:

[0091]

[0092] S300, testing the estriol derivatives and underivatized estriol produced by the reaction using an LC-MS detection method. see Figure 6 , Figure 7 , Figure 8 and Figure 9 In the negative ion mode, the full-scan mass spectrogram is used to detect whether there is derivatized and underivatized estriol in the sample to be tested, and then the single-ion monitoring ion chromatogram is used for quantitative testing. The diazo derivatization method can significantly improve the signal of estriol single ion detection scanning quantitative test.

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Abstract

The present invention provides a method for detecting an aromatic compound, comprising: providing a diazonium salt; using the diazonium salt to perform a diazonium derivatization process on an aromatic compound in a sample to be tested to obtain a diazonium derivatized product; and The diazo derivatized products were detected by liquid chromatography-mass spectrometry. The present invention also provides a kit for detecting aromatic compounds by liquid chromatography-mass spectrometry, including a diazo derivatization reagent, wherein the diazo derivatization reagent includes a reagent for diazotizing aromatic compounds in a sample to be tested. At least one of the derivatized diazonium salt or the raw materials for the preparation of the diazonium salt.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to a detection method and a kit for aromatic compounds. Background technique [0002] Liquid chromatography-mass spectrometry (LC-MS) detection method with structural resolution ability has gradually become one of the main methods for quantitative detection of biologically active substances. The application of LC-MS detection methods in the field of clinical detection is also becoming more and more extensive, especially for the quantitative testing of small molecular compounds. In the practical application of clinical detection, when the concentration of the substance to be tested is low, the sensitivity requirements for the detection method will increase. When the blood sample volume is limited, for example, the sample is a micro blood sample (1 μl-10 μl blood volume) or a dried blood sample (DBS), the sensitivity of the detection method will limit the feasibility of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/72
CPCG01N33/50G01N30/06G01N2030/067G01N2030/8813G01N30/88G01N2560/00C07J41/0005C07J1/007G01N30/7233G01N30/8679G01N2030/027G01N33/743Y10T436/24Y10T436/212
Inventor 骆亦奇
Owner APPLIN BIOTECH CO LTD
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