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A kind of composite stabilizer used in complement c3 assay kit and application thereof

A composite stabilizer and kit technology, applied in biological testing, measuring devices, instruments, etc., can solve problems such as the stability description of kits, and achieve the effects of ensuring accuracy, simple preparation and low cost

Active Publication Date: 2020-04-28
ZHONGSHAN CHUANGYI BIOCHEM ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patents CN201610431532.X and CN201510226758.1 provide a latex immunoturbidimetric complement C3 kit and its preparation method, but no stabilizer is added, and the stability of the kit is not explained; patent CN201510226757.7 provides a A latex-enhanced immunoturbidimetric complement C3 detection method also does not add any stabilizer, and does not explain the stability of the kit

Method used

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  • A kind of composite stabilizer used in complement c3 assay kit and application thereof
  • A kind of composite stabilizer used in complement c3 assay kit and application thereof
  • A kind of composite stabilizer used in complement c3 assay kit and application thereof

Examples

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Effect test

Embodiment 1

[0034] A compound stabilizer for complement C3 assay kit, the content of the compound stabilizer components is: 10g / L bovine serum albumin, 10g / L propylene glycol, 1g / L Triton X-100, 15g / L sucrose , 2g / L liquid biological preservative Proclin300.

[0035] The preparation method of above-mentioned composite stabilizer, comprises the following steps:

[0036] (1) Add bovine serum albumin, propylene glycol, and sucrose that have been weighed successively in the dissolving bucket, add an appropriate amount of water and stir to make it dissolve completely;

[0037] (2) Then add Triton X-100 to fully emulsify the above raw materials;

[0038] (3) After the emulsification is complete, add Proclin300 and sufficient water, stir to dissolve completely, seal the reaction barrel with a lid, and filter to obtain the product.

Embodiment 2

[0040] A compound stabilizer for complement C3 assay kit, the content of the compound stabilizer components is: 1g / L bovine serum albumin, 2g / L propylene glycol, 1g / L Triton X-100, 2g / L sucrose , 0.1g / L liquid biological preservative Proclin300.

[0041] The preparation method of above-mentioned composite stabilizer, comprises the following steps:

[0042] (1) Add bovine serum albumin, propylene glycol, and sucrose that have been weighed successively in the dissolving bucket, add an appropriate amount of water and stir to make it dissolve completely;

[0043] (2) Then add Triton X-100 to fully emulsify the above raw materials;

[0044] (3) After the emulsification is complete, add Proclin300 and sufficient water, stir to dissolve completely, seal the reaction barrel with a lid, and filter to obtain the product.

Embodiment 3

[0046] A compound stabilizer for complement C3 assay kit, the content of the compound stabilizer components is: 5g / L bovine serum albumin, 2.5g / L propylene glycol, 1g / L Triton X-100, 1.5g / L Sucrose, 0.2g / L liquid biological preservative Proclin300.

[0047] The preparation method of above-mentioned composite stabilizer, comprises the following steps:

[0048] (1) Add bovine serum albumin, propylene glycol, and sucrose that have been weighed successively in the dissolving bucket, add an appropriate amount of water and stir to make it dissolve completely;

[0049] (2) Then add Triton X-100 to fully emulsify the above raw materials;

[0050](3) After the emulsification is complete, add Proclin300 and sufficient water, stir to dissolve completely, seal the reaction barrel with a lid, and filter to obtain the product.

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Abstract

The invention discloses a composite stabilizer for a C3 (complement 3) determination kit. The composite stabilizer comprises the following raw materials by weight: 1-5% of a blocking agent, 1-2.5% ofsucrose, 1-2% of a surfactant, 1-1.5% of an emulsifier, 0.1-0.2% of a liquid biological preservative, and the balance purified water. The composite stabilizer can be used in a C3 determination kit, and the dosage accounts for 1-3% of the volume fraction of reagent in the kit. The composite stabilizer provided by the invention has the characteristics of simple preparation and convenient operation,ensures the accuracy of the kit detection result, also can make the C3 determination kit more stable, and prolongs the preservation time of the kit.

Description

technical field [0001] The invention belongs to the field of in vitro diagnosis, in particular to a composite stabilizer used in a complement C3 assay kit, a preparation method and application thereof. Background technique [0002] Complement (complement, C) is a group of proteins with zymogen activity existing in human and vertebrate serum and interstitial fluid. C3 is the most abundant complement component in serum, with a molecular weight of 195,000. It is a kind of β-globulin synthesized mainly by macrophages, monocytes, lymphoid tissues, bone marrow, peritoneum and liver. Under the action of C3 convertase, it is cleaved It forms two fragments, C3a and C3b, which play an important role in both the classical activation pathway and the alternative activation pathway of complement. [0003] The dynamic change of complement has been paid more and more attention in clinic. The increase or decrease of complement C3 concentration in serum is often related to the condition of t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/531
Inventor 何林辉崔海林曾晓君
Owner ZHONGSHAN CHUANGYI BIOCHEM ENG
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