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Method for fast inducing spermiation and ovulation of pinctada maxima parent oysters

A kind of large pearl oyster, fast technology, applied in fish farming, application, climate change adaptation and other directions, to achieve the effect of high maturity, simple technology, easy to promote

Inactive Publication Date: 2018-01-23
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the shortcomings of the method for obtaining fertilized eggs by artificial seedling cultivation of Pinctada maxima, the present invention provides a method with high discharge rate, remarkable economic benefit and social benefit, which can be used in large-scale artificial seedling cultivation of Pinctada maxima to induce ovulation and oviposition of parent shellfish Methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Adopt the following steps to realize the present invention:

[0019] Artificially Inducing Ejaculation and Oviposition of Natural Pinctada maxima by Ultraviolet Irradiation of Seawater

[0020] (1) Collect 3-4-year-old natural pearl oysters with a shell height of 14-20cm and full gonads as broods. There are 6 male oysters and 30 female oysters, ♀:♂=5:1; Transport it back to the farm, clean the attachments on the shell surface, put it into a plastic basket, and disinfect it with 20-30mg / L potassium permanganate solution for 1-2min;

[0021] (2) Use a 20-watt ultraviolet lamp to irradiate and filter seawater. The seawater depth is 80cm. The ultraviolet lamp is 20cm away from the water surface. The irradiation dose is 100 milliwatts per liter of seawater. , reflecting light into the sea water;

[0022] (3) Flowing water stimulation: directly hang and raise the broiler shellfish in step (1) without drying in the shade. The water depth of the nursery pool is 80 cm, hang th...

Embodiment 2

[0025] Ultraviolet irradiating seawater to artificially induce spermatogenesis and spawning in artificially cultured Pinctada maxima

[0026] (1) Select the artificially cultured Pearl oyster with a shell age of 3-4 years and a shell height of 16-20cm with full gonads as brood shells, ♀:♂=6:1, clean the attachments on the shell surface, and put them into a plastic basket , disinfect with 20-30mg / L potassium permanganate solution for 1-2min;

[0027] (2) Use a 40-watt ultraviolet lamp to irradiate and filter seawater. The seawater depth is 100cm, the ultraviolet lamp is 40cm away from the water surface, and the irradiation dose is 200mW·hour / liter of seawater;

[0028] (3) Stimulation by running water: directly hang and raise the broodstock described in step (1) in the nursery pond without drying in the shade. The water depth of the nursery pond is 100 cm. Stimulate with running water on the top, after 15 minutes of running water stimulation, broodlings start to ejaculate and ...

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Abstract

The invention discloses a method for fast inducing spermiation and ovulation of pinctada maxima parent oysters, and belongs to the technical field of aquaculture. The method comprises the steps that natural oysters at the age of 3-4 or artificially-cultured pinctada maxima is selected as the parent oysters, shells of the parent oysters are complete, the activity is high, gonads are full, and the shell is washed after attachments on the surfaces of the shells are eliminated; the proportion of the female oysters to the male oysters of the parent oysters is 4-6:1, the parent oysters are placed ina plastic frame or a mesh cage and hung in an oviposition pool for culture, running water stimulation is conducted by means of filtered seawater irradiated by ultraviolet light, the irradiation doseis 100-200 milliwatt.hour / litre of the seawater, and 15-60 min later, the parent oysters start spermiation and ovulation. According to the method, running water stimulation is conducted on the pinctada maxima parent oysters for spermiation and ovulation by means of the filtered seawater irradiated by the ultraviolet light, sperms and eggs released by the parent oysters have high maturity, the fertilization rate is high, larvae are easily cultured, and the survival rate is high; the method is suitable for induction of large-scale artificial seedling culture of the pinctada maxima, popularization in a production unit is easy, and significant social benefits, economical benefits and ecological benefits are provided.

Description

technical field [0001] The invention relates to a method for catalyzing rapid induction of ejaculation and oviposition of broodfish of Pinctada grandis, belonging to the technical field of aquaculture. Background technique [0002] Large mother-of-pearl Pinctada maxima Jameson, commonly known as white butterfly shell, is distributed in Australia, the Philippines, Malaysia, Indonesia and my country's Leizhou Peninsula, Hainan, Xisha Islands and other sea areas. It is mainly used to cultivate large seawater pearls with a diameter greater than 10 mm. my country's pearl oyster artificial cultivation began in the 1970s, and free pearls were successfully cultivated in 1978, but it has not been industrialized so far. The domestic consumption of pearl oyster still depends on imports. The bottleneck affecting the development of the pearl oyster pearl industry lies in the very low survival rate (<1%) during the cultivation period in the seedling sea area, which fails to provide s...

Claims

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Application Information

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IPC IPC(8): A01K61/54
CPCY02A40/81
Inventor 梁飞龙邓岳文王庆恒杜晓东
Owner GUANGDONG OCEAN UNIVERSITY