Edible fungus strain purification and rejuvenation technology
A technology for purifying and rejuvenating and edible fungi, applied in the directions of fungi, microorganisms, biochemical equipment and methods, etc., can solve the problems of aggravating the degradation process, slow growth of mycelium, decreasing yield, etc., improving yield and quality, and simplifying the preparation method. , the effect of improving vitality
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Embodiment 1
[0019] The purification and rejuvenation of shiitake mushroom strains comprises the following steps,
[0020] (1) Three transfers of mycelial tips: put the degenerated edible fungus strains to be purified and rejuvenated into the first culture medium, and when the diameter of the colony reaches 2-3cm, the cut tip is transplanted into the second culture medium , when the diameter of the colony reaches 2-3cm again, the cutting tip is transplanted and placed in the third medium, and when the diameter of the colony reaches 2-3cm again, the cutting tip is transplanted and placed in the fourth medium; the medium is Regular medium. In step (1), the temperature of the strains in each medium is 31°C.
[0021] (2) Antibiotic culture: when the diameter of the strain colony transplanted for the last time in step (1) reaches 2-3 cm, move the strain to the shake flask culture solution, and add penicillin to the shake flask culture solution, the resulting The addition of penicillin is 2,00...
Embodiment 2
[0027] Purification and Rejuvenation of Strains of Pleurotus ostreatus
[0028] (1) Three transfers of mycelial tips: put the degenerated edible fungus strains to be purified and rejuvenated into the first culture medium, and when the diameter of the colony reaches 2-3cm, the cut tip is transplanted into the second culture medium , when the diameter of the colony reaches 2-3cm again, the cutting tip is transplanted and placed in the third medium, and when the diameter of the colony reaches 2-3cm again, the cutting tip is transplanted and placed in the fourth medium; the medium is Regular medium. In step (1), the temperature of the strains in each medium is 30°C.
[0029] (2) Antibiotic culture: when the diameter of the strain colony transplanted for the last time in step (1) reaches 2-3 cm, move the strain to the shake flask culture solution, and add penicillin to the shake flask culture solution, the resulting The addition of penicillin is 2,000,000 units / L, and the bacteri...
Embodiment 3
[0034] Example 3 Purification and rejuvenation of Auricularia trichotillotype
[0035] (1) Three transfers of mycelial tips: put the degenerated edible fungus strains to be purified and rejuvenated into the first culture medium, and when the diameter of the colony reaches 2-3cm, the cut tip is transplanted into the second culture medium , when the diameter of the colony reaches 2-3cm again, the cutting tip is transplanted and placed in the third medium, and when the diameter of the colony reaches 2-3cm again, the cutting tip is transplanted and placed in the fourth medium; the medium is Regular medium. In step (1), the temperature of the strains in each medium is 32°C.
[0036] (2) Antibiotic culture: when the diameter of the strain colony transplanted for the last time in step (1) reaches 2-3 cm, move the strain to the shake flask culture solution, and add penicillin to the shake flask culture solution, the resulting The addition of penicillin is 2,000,000 units / L, and the ...
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