Application of miR-505-3p in diagnosis, prognosis and treatment of PCa (prostate cancer) bone metastasis

A technology for prostate cancer and bone metastasis, applied in the field of molecular biology, can solve the problems of no miR-505-3p research report, lack of treatment methods, death of prostate cancer patients, etc.

Active Publication Date: 2018-01-26
THE SECOND AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although substantial progress has been made in the treatment of early prostate cancer, there is a lack of effective treatment after prostate cancer metastasis, which is an important cause of death in prostate cancer patients, especially bone metastasis
There is no research report on the relationship between miR-505-3p and bone metastasis of prostate cancer

Method used

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  • Application of miR-505-3p in diagnosis, prognosis and treatment of PCa (prostate cancer) bone metastasis
  • Application of miR-505-3p in diagnosis, prognosis and treatment of PCa (prostate cancer) bone metastasis
  • Application of miR-505-3p in diagnosis, prognosis and treatment of PCa (prostate cancer) bone metastasis

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0031] Experimental Example 1. Differential expression of miR-505-3p in prostate cancer bone metastases and non-bone metastases

[0032] RNA extraction, reverse transcription, and real-time quantitative RT-PCR detection of miR-505-3p: Total RNA was extracted from all tissues using TRIzol Lysis Solution (LifeTechnologies Company) instructions, and reverse transcription was performed according to the instructions of Revert Aid First Strand cDNASynthesis Kit (Thermo Fisher Company) , using conventional operations for reverse transcription; using U6 as an internal reference, using the 2-ddCt relative quantitative method for fluorescence quantitative detection, and calculating the relative expression of miR-505-3p; among them, the detection of U6 and miR-505-3p expression The primers were synthesized and purified by Guangzhou Ruibo Biotechnology Co., Ltd.

[0033] The inventors found that miR-505-3p was differentially expressed in prostate cancer bone metastases and non-bone metast...

experiment example 2

[0034] Experimental example 2, miR-505-3p is low expressed in prostate cancer cell lines with lymph node metastasis, brain metastasis and bone metastasis

[0035] Normal human prostate epithelial cells RWPE-1 and human prostate cancer cell lines DU145, LNCaP, 22Rv1, PC-3, VCaP, C4-2B were cultured, total RNA was extracted, reverse transcription and real-time quantitative RT-PCR were used to detect miR-505- 3p, the method is the same as Experimental Example 1. The result is as figure 2As shown, compared with the normal human prostate epithelial cell line RWPE-1, miR- 505-3p low expression, in vitro experiments proved that miR-505-3p is associated with prostate cancer metastasis.

experiment example 3

[0036] Experimental example 3. GSEA analysis verifies that the low expression of miR-505-3p is positively correlated with the metastasis of prostate cancer

[0037] The inventors utilized gene set enrichment analysis (GSEA), such as image 3 As shown, it was also found that the low expression of miR-505-3p was positively correlated with the metastasis propensity of prostate cancer.

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Abstract

The invention discloses an application of miR-505-3p in diagnosis, prognosis and treatment of PCa (prostate cancer) bone metastasis. Research finds that miR-505-3p has low expression in PCa tissue ofbone metastasis and in PCa cell lines of metastasis, further, miR-505-3p is in negative correlation with N and M clinical staging and Gleason grading of PCa patients, and low expression of miR-505-3pindicates lower survival rate in bone-metastasis-free progress; besides, up-regulated miR-505-3p inhibits migration and invasion of the PCa cell lines of bone metastasis. Above all, a reagent for detecting expression quantity of miR-505-3p can be used for diagnosis and prognosis of PCa bone metastasis, and substances capable of up-regulating the expression quantity of miR-505-3p are expected to become a treatment drug for inhibiting PCa bone metastasis.

Description

technical field [0001] The invention belongs to the field of molecular biology, and more specifically relates to the application of miR-505-3p in the diagnosis, prognosis and treatment of prostate cancer bone metastasis. Background technique [0002] Prostate cancer (PCa) is one of the most common malignant tumors of the male genitourinary system. Although substantial progress has been made in the treatment of early prostate cancer, there is a lack of effective treatment after prostate cancer metastasis, which is an important cause of death in prostate cancer patients, especially bone metastasis. Therefore, the early diagnosis of prostate cancer metastasis is the key to clinical diagnosis, treatment and prognosis. Finding a new marker for predicting prostate cancer metastasis is of great help to the treatment of prostate cancer. [0003] miRNA is a small non-coding RNA that inhibits the translation of mRNA by binding to the 3' untranslated region of mRNA. miRNA plays an im...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
Inventor 黄帅唐欲博瓦庆德郭远清彭新生
Owner THE SECOND AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV
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