Preparation method of feed capable of promoting growth of fish bred in fresh water
A freshwater breeding and feed technology, applied in animal feed, climate change adaptation, additional food elements, etc., can solve the problems of reducing the nutritional content of raw materials, expensive imported fishmeal, and little promotion value, so as to improve fish health and reduce Indigestion and allergic reactions, the effect of reducing the chance of fish diseases
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Embodiment 1
[0022] A method for preparing a feed for promoting the growth of freshwater aquaculture fish, comprising the following steps:
[0023] 1) Dissolve 3 parts of tea polyphenol powder in 60 parts of absolute ethanol, ultrasonically dissolve, add 8 parts of egg yolk lecithin and 2.0 parts of cholesterol mixed solution in water bath and stir to dissolve, quickly inject the above lipid ethanol solution to 80 parts with a syringe Na at pH 7.2 2 HPO 4 -KH 2 PO 4 In the buffer solution, keep the pH constant, stir and hydrate at 35°C for 15 minutes, and obtain tea polyphenol nanoliposomes by rotary evaporation under reduced pressure; Atractylodes macrocephala, 3 parts of Radix Astragali, and 10 parts of Fructus Sinensis are crushed with a pulverizer, passed through a 80-mesh sieve, and made into a compound Chinese medicine preparation; weigh the compound Chinese medicine preparation, wrap it in absorbent cotton gauze, add 8 times the amount of distilled water, soak for 1 hour, and boi...
Embodiment 2
[0025] The preparation method of the feed for promoting the growth of freshwater aquaculture fish includes: preparation of liposome, preparation of traditional Chinese medicine preparation, modification of bran, fermented soybean meal, and compounding, specifically including the following steps:
[0026] 1) Preparation of liposomes: Dissolve 4 parts of tea polyphenol powder, 10 parts of egg yolk lecithin and 2.5 parts of cholesterol in 75 parts of absolute ethanol, and quickly inject the above lipid ethanol solution to 100 parts with a pH of 7.5 using a syringe Na 2 HPO 4 -KH 2 PO 4 In the buffer solution, keep the pH constant, stir and hydrate at 38°C for 25 minutes to obtain a suspension of tea polyphenol nanoliposomes, and the suspension is subjected to reduced-pressure rotary evaporation to remove residual ethanol to obtain tea polyphenol nanolipids body; tea polyphenols have antioxidant properties, detoxification, anti-radiation effects, and have the effect of scavengi...
Embodiment 3
[0032] The preparation method of the feed for promoting the growth of freshwater aquaculture fish includes: preparation of liposome, preparation of traditional Chinese medicine preparation, modification of bran, fermented soybean meal, and compounding, specifically including the following steps:
[0033] Preparation of liposomes: Dissolve 4 parts of tea polyphenol powder in 65 parts of absolute ethanol, ultrasonically dissolve, add 10 parts of egg yolk lecithin and 2.2 parts of cholesterol mixed solution in a water bath and stir to dissolve, use a syringe to rapidly dissolve the lipid ethanol solution Inject into 90 parts of Na with a pH of 7.4 2 HPO 4 -KH 2 PO 4 In the buffer solution, keep the pH constant, stir and hydrate at 37°C for 20 minutes to obtain a suspension of tea polyphenol nanoliposomes, and the suspension is subjected to reduced-pressure rotary evaporation to remove residual ethanol to obtain tea polyphenol nanolipids body; tea polyphenols have antioxidant p...
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