Compounds, conjugates, kits and their use in the detection of estradiol
A technology of compounds and conjugates, which is applied in the field of analysis, can solve problems such as estradiol derivatives to be developed, and achieve the effect of being easy to popularize, apply and obtain
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Embodiment 1
[0058] In this embodiment, compound shown in formula (2) is synthesized according to the following process:
[0059]
[0060] The synthetic route is as follows:
[0061]
[0062] Concrete synthetic steps are as follows:
[0063] 1. Synthesis of compound 2:
[0064] Feed 2.72g (0.01mol) of 17β-estradiol (compound 1) into a 100mL dry and cooled reaction flask, add 40mL of dimethylformamide (DMF), stir to dissolve, then add 3.14g (0.03mol) of anhydrous potassium carbonate , Under stirring, 2.17g (0.012mol) methyl 4-bromobutyrate was slowly added dropwise. After the dropwise addition was completed, the reaction was carried out for 4 hours. The reaction solution was poured into ice water, extracted three times with ethyl acetate, the combined organic layers were washed three times with saturated brine, then dried over anhydrous sodium sulfate and concentrated to obtain 4.7 g of crude compound 2.
[0065] MS: positive ion peak: 373.6, 395.6 (+Na peak); negative ion peak: 3...
Embodiment 2
[0070] In this embodiment, compound shown in formula (3) is synthesized according to the following process:
[0071]
[0072] The synthetic route is as follows:
[0073]
[0074] Concrete synthetic steps are as follows:
[0075] 1. Synthesis of compound 5:
[0076] Feed 2.70g (0.01mol) of estrone (compound 4) in a 100mL dry and cooled reaction flask, add 40mL of DMF, stir to dissolve, then add 3.14g (0.03mol) of anhydrous potassium carbonate, and slowly add 2.17g ( 0.012mol) methyl 4-bromobutyrate. After the dropwise addition was completed, the reaction was carried out for 4 hours. The reaction solution was poured into ice water, extracted three times with ethyl acetate, the combined organic layers were washed three times with saturated brine, then dried over anhydrous sodium sulfate and concentrated to obtain 4.6 g of crude compound 5.
[0077] MS: positive ion peak: 371.5, 393.5 (+Na peak); negative ion peak: 369.5.
[0078] 2. Synthetic formula (3) compound:
[...
Embodiment 3
[0082] In this embodiment, compound shown in formula (4) is synthesized according to the following process:
[0083]
[0084] synthetic route
[0085]
[0086] Concrete synthetic steps are as follows:
[0087] 1. Synthesis of compound 8:
[0088] Feed 2.88g (0.01mol) of estriol (compound 7) in a 100mL dry and cooled reaction flask, add 40mL of DMF, stir to dissolve, add 3.14g (0.03mol) of anhydrous potassium carbonate, and slowly add 2.17g (0.012 mol) methyl 4-bromobutyrate. After the dropwise addition was completed, the reaction was carried out for 4 hours. The reaction solution was poured into ice water, extracted three times with ethyl acetate, the organic layers were combined, washed three times with saturated brine, dried over anhydrous sodium sulfate, and concentrated to obtain 4.9 g of crude compound 8.
[0089] MS: positive ion peak: 389.7, 411.7 (+Na peak); negative ion peak: 387.8.
[0090] 2. Synthetic formula (4) compound:
[0091] Feed 4.9 g of the cru...
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