Liquid drop temperature circular reaction equipment

A technology of temperature cycling and reaction equipment, applied in series/parallel reactions, heating or cooling equipment, biochemical equipment and methods, etc. The effect of occupying space, reducing test cost, and shortening test cycle

Inactive Publication Date: 2018-02-09
ACUDX INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The existing technology has the following disadvantages: the digital PCR reaction time is long, and the equipment occupies a large space and is complicated

Method used

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  • Liquid drop temperature circular reaction equipment

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Embodiment Construction

[0044]Specific implementations of the present invention will be described below. It should be noted that in the process of specific descriptions of these implementations, for the sake of concise description, it is impossible for this specification to describe all the features of the actual implementations in detail. It should be understood that, in the actual implementation process of any embodiment, just like in the process of any engineering project or design project, in order to achieve the developer's specific goals and to meet system-related or business-related constraints, Often a variety of specific decisions are made, and this can vary from one implementation to another. In addition, it will be appreciated that while such development efforts may be complex and lengthy, the technology disclosed in this disclosure will be Some design, manufacturing or production changes based on the content are just conventional technical means, and should not be interpreted as insuffici...

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Abstract

The invention relates to liquid drop temperature circular reaction equipment which comprises a spiral colloid liquid drop guide pipe, a liquid drop flowing driving pump and a temperature control piece. A colloid liquid drop suspension is connected to an inlet end of the spiral colloid liquid drop guide pipe, and the liquid drop flowing driving pump is connected to an outlet end or an inlet end ofthe spiral colloid liquid drop guide pipe, so that colloid liquid drops driven by the liquid drop flowing driving pump flow through the spiral colloid liquid drop guide pipe; the temperature control piece is arranged on the outer side or the inner side of the spiral colloid liquid drop guide pipe and comprises at least two different temperature control areas, so that when the colloid liquid dropsflow through the spiral colloid liquid drop guide pipe, the colloid liquid drops flow through different temperature control areas. The liquid drop temperature circular reaction equipment has the advantages that complexity of the equipment is lowered, the equipment can be downsized, micro-scale liquid drop PCR is realized, and digital PCR testing cost is lowered.

Description

technical field [0001] The invention relates to liquid droplet temperature cycle reaction equipment. Background technique [0002] At present, the droplet temperature cycle reaction (for example, droplet digital PCR reaction) in the prior art is mainly carried out in test tubes and temperature control blocks on PCR (polymerase chain reaction) instruments. The temperature cycle is carried out by cyclically adjusting the temperature of the temperature control block. After the cyclic reaction is over, another detection instrument is needed for the quantitative detection of the droplets. [0003] Digital PCR is a technique for the absolute quantification of nucleic acid molecules. There are currently two quantitative methods for nucleic acid molecules based on PCR: real-time fluorescence quantitative PCR, which is based on the Ct value, and the Ct value refers to the number of cycles corresponding to the fluorescence value that can be detected; digital PCR, which is the latest...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/38C12M1/34B01J19/00
CPCB01J19/0046B01L3/5085B01L3/50851B01L3/545B01L7/52B01L7/525B01L2200/10B01L2300/1805B01L2200/0673B01L2400/0487C12Q1/686B01L3/502784B01L2300/0832B01L2300/1827B01L2300/1861B01L2300/0627B01L2300/0848
Inventor 王张敏程传芬李喜彤
Owner ACUDX INC
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