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Fluorescence detection method and system

A technology for detecting signal and fluorescence emission, which is applied in the field of fluorescence detection and system, and can solve problems such as limited utility and crosstalk

Active Publication Date: 2021-08-24
MOLECULAR DEVICES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these systems suffer from crosstalk as emission from one lanthanide flows into the detection channel of the other lanthanide
This limits the utility of these methods to only one channel that is truly sensitive and the other channel is limited by the background signal from the second substance

Method used

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  • Fluorescence detection method and system
  • Fluorescence detection method and system
  • Fluorescence detection method and system

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Embodiment

[0120] Protein detection and characterization are important tasks in pharmaceutical and clinical research. For example, protein detection and characterization can provide information about up- and down-regulation of proteins in cells, phosphorylation during cell signaling, and expression of transfected proteins. Various techniques have been developed for protein analysis, including plate reader-based enzyme-linked immunosorbent assay (ELISA), sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and Spot or bead based capture systems utilizing luminescence readouts. However, improvements in analytical methods to detect and quantify proteins are important to provide better tools to aid in the understanding of disease mechanisms.

[0121] Many luminescent probes have been developed to detect and analyze proteins. Probes are typically linked to primary or secondary antibodies, which then selectively bind the protein of interest. These probes can be fluorescent ...

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Abstract

The present invention relates to multiplex fluorescence detection, including time-resolved fluorescence (TRF) detection. A combination of spectral and temporal differences in fluorescence emission and spectral differences in excitation is used to enhance the ability to separate signals from assays of multiple fluorescent labels. Different types of labels can be used, including upconverting phosphors as well as lanthanide and transition metal chelates. The method can be implemented in an optical plate reader comprising a cartridge-based multimodal reader.

Description

[0001] related application [0002] This application claims priority to US Patent Application Serial No. 14 / 682,026, filed April 8, 2015, which is hereby incorporated by reference in its entirety. technical field [0003] The present invention generally relates to methods, devices and systems for fluorescence-based detection or measurement of samples, particularly multiplex detection using different fluorophores. The present invention can implement different types of techniques for fluorescence-based detection, including common fluorescence detection (FD) and time-resolved fluorescence (TRF) detection. Background technique [0004] Protein detection and characterization are important tasks in pharmaceutical and clinical research. Chemiluminescence (CL) is a common method for protein detection in biochemical assays, or for detection of proteins in surface-bound and spatially separated proteins. An example of the latter is the method of sodium dodecyl sulfate-polyacrylamide ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6408G01N2021/6441G01N2021/6421G01N2021/6419G01N2201/0245
Inventor 瓦尼哈·图拉斯拉曼迈克尔·卡茨林格埃文·F·克伦威尔安妮格瑞特·施拉姆
Owner MOLECULAR DEVICES