Stable extracting method for anthocyanin in hongye poplar leaves
An anthocyanin and extraction method technology, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of reducing the reliability of results, large experimental errors, and large losses, and achieve easy control and maintenance Stable, less time-consuming effect
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[0024] A method for extracting and determining the stability of anthocyanins in poplar leaves, which specifically includes the following steps:
[0025] (1) Preparation of materials:
[0026] Collect the 3-5 leaves from the top of the two-year-old red-leaved poplar seedlings. The collected sample leaves are immediately stored in an ice box and taken to the laboratory. After being rinsed with tap water, rinse with distilled water for 1-2 times and dry in the air. Remove the petioles and veins, cut into pieces with a diameter of less than 0.5mm, mix well, and refrigerate at 5°C for later use;
[0027] (2) Selection of extraction solvent and calculation method:
[0028] Weigh 1.0g of populus red leaves obtained in step (1), put them in a 50ml Erlenmeyer flask, add 10ml of different extraction solvents; 0.1mol / L hydrochloric acid, 1% hydrochloric acid-methanol, 1% hydrochloric acid-ethanol, 0.1% hydrochloric acid-60% methanol, 0.1% hydrochloric acid-60% ethanol, seal the bottle ...
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