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Polyphosphate kinase gene and applications thereof in sewage dephosphorization

A technology of polyphosphate and kinase, applied in the field of genetic engineering

Active Publication Date: 2018-03-02
HAINAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the research on phosphorus-accumulating bacteria in mangrove soil. Mangroves are tidal flat wetland biological communities, which have been subjected to periodic inundation by seawater for a long time. Highly consistent and rich in phosphorus-accumulating microbial resources

Method used

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  • Polyphosphate kinase gene and applications thereof in sewage dephosphorization
  • Polyphosphate kinase gene and applications thereof in sewage dephosphorization
  • Polyphosphate kinase gene and applications thereof in sewage dephosphorization

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Embodiment Construction

[0044] In order to facilitate a further understanding of the present invention, the examples provided below illustrate it in more detail. However, these examples are only for a better understanding of the invention and are not used to limit the scope or implementation principle of the invention, and the embodiments of the invention are not limited to the following content.

[0045] Whole Genome Sequence Data Analysis

[0046] 1) Fragmentation of genomic DNA: Use Covaris M220 to decompose genomic DNA into several fragments of about 300-500bp in size;

[0047] 2) Library construction: A&B adapters were connected at both ends of the DNA fragments, and then screened to remove the self-connected fragments of the adapters. The fragments were screened by agarose gel electrophoresis, and the fragments with one end of the A adapter and the other end of the B adapter were retained. Denature DNA to produce single-stranded DNA fragments;

[0048] 3) Bridge PCR: One end of the DNA fragme...

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PUM

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Abstract

The invention relates to a polyphosphate kinase gene and applications of the polyphosphate kinase gene in sewage dephosphorization, and particularly relates to three novel polyphosphate kinase genes,namely, ppk33, ppk44 and ppk73 which are respectively provided with nucleotide sequences as shown in SEQ NO 1, SEQ NO2 and SEQ NO.3. An engineering strain prepared by adopting the polyphosphate kinasegenes ppk33, ppk44 and ppk73 can be used for removing phosphorus element in sewage, wherein the strain M15 / pQE30a-ppk44T has the phosphorus removal amount being 7 times of that of a corresponding contrast strain, and the phosphorus removal efficiency achieves 70% after 10h.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and in particular relates to a polyphosphate kinase gene and its application in phosphorus removal from sewage. Background technique [0002] Eutrophication of water bodies has become a global problem and is getting worse day by day. In recent years, a large amount of nitrogen, phosphorus, potassium and other elements have been discharged into various water bodies far faster than their consumption speed. Breaking through, water eutrophication has become a global problem and is getting worse day by day (Yang X.E, Wu X., Hao H.L., He Z.l.Mechanisms and assessment of water eutrophication[J].Journal of ZhejiangUniversity B,2008,3(3): 197-209). Among all the nutrient elements, phosphorus is particularly important to the growth of algae. With phosphorus as the limiting factor, when the total phosphorus concentration exceeds 0.1mg / L, algae will overgrow (Lin Xiao, Liu Qing, Xu Houkun. Phosphorus in e...

Claims

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Application Information

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IPC IPC(8): C12N15/54C12N9/12C12N1/21C02F3/34C02F101/10
CPCC02F3/34C02F2101/105C12N9/1229C12Y207/04001
Inventor 王锐萍张文飞伍思宇张起畅殷浩能吴红萍金映虹
Owner HAINAN NORMAL UNIVERSITY