Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing rare ginseng saponins by utilizing schizophyllum biotransformation ginsenosides

A technology of rare ginseng saponins and ginsenosides, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve problems such as insecurity, and achieve the effect of improving health care and medicinal efficacy

Active Publication Date: 2021-02-26
JILIN AGRICULTURAL UNIV
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It overcomes the shortcomings of using unsafe bacteria and molds that cannot be used in food and pharmaceutical production as fermentation strains in the past

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing rare ginseng saponins by utilizing schizophyllum biotransformation ginsenosides
  • Method for preparing rare ginseng saponins by utilizing schizophyllum biotransformation ginsenosides
  • Method for preparing rare ginseng saponins by utilizing schizophyllum biotransformation ginsenosides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046]Schizophyllum schizophyllum was inoculated into the liquid fermentation medium at 5% of the inoculum, and the pH of the medium was 8, ginsenoside Rb1The feed rate is 5g / L, the rotation of the shaker is 120 r / min, and the fermentation is cultured at 20°C for 4 days. After the fermentation, the bacteria liquid was filtered, and the hyphae were ultrasonically extracted 3 times with 80% ethanol, each for 30 minutes. The extract was recovered under reduced pressure at 40°C to recover the solvent, concentrated into an aqueous solution, and then the concentrated extract was combined with the bacterial solution; separated with macroporous resin D101, eluted with a gradient of 20%-90% ethanol, and detected by thin-layer chromatography , Collect and combine the fraction solutions with the same Rf value as the ginseng rare saponins positive reference substance, recover the solvent under reduced pressure, and use preparative liquid chromatography to separate and prepare to obtain ginsenos...

Embodiment 2

[0048]Schizophyllum schizophylla was inoculated into the liquid fermentation medium at 10% of the inoculum, the pH of the medium was 7, and ginsenoside Rb2The feed rate is 10g / L, the rotation of the shaker is 140 r / min, and the fermentation is cultured at 22°C for 5 days. After the fermentation, the bacterial liquid was filtered, and the hyphae were ultrasonically extracted 4 times with 80% ethanol, each for 30 minutes. The solvent was recovered from the extract under reduced pressure at 50°C and concentrated to an aqueous solution. Then the concentrated extract was combined with the bacterial solution, separated with macroporous resin D101, eluted with a gradient of 20%-90% ethanol, detected by thin layer chromatography, collected and merged with the same Rf value of the ginseng rare saponin positive control substance The fraction solution, the solvent was recovered under reduced pressure, and separated and prepared by preparative liquid chromatography to obtain compound O and comp...

Embodiment 3

[0050]Schizophyllum schizophylla was inoculated into the liquid fermentation medium at 15% of the inoculum, the pH of the medium was 6, the feed rate of ginsenoside Rc was 20g / L, the rotation of the shaker was 160 r / min, and the fermentation was carried out at 25°C. Cultivate for 6 days. After the fermentation, the bacterial liquid was filtered, and the hyphae were ultrasonically extracted 5 times with 80% ethanol, each for 30 minutes. The solvent was recovered from the extract under reduced pressure at 60°C and concentrated to an aqueous solution. Then the concentrated extract was combined with the bacterial solution, separated with macroporous resin D101, eluted with a gradient of 20%-90% ethanol, detected by thin layer chromatography, collected and merged with the same Rf value of the ginseng rare saponin positive control substance The fraction solution is recovered under reduced pressure and the solvent is separated and prepared by preparative liquid chromatography to obtain com...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for preparing rare ginsenosides by using Schizophyllum biotransformation of ginsenosides. The edible and medicinal fungus Schizophyllum is used as the fermentation strain and inoculated into a liquid fermentation medium at an inoculum size of 5%-30%. , the pH of the medium is 2-9, the feeding amount of panaxadiol group saponins is 1-60g / L, the rotation speed of the shaker is 10-300r / min, and the fermentation is carried out at 15-40°C for 2-10 days. Schizophyllum in the present invention can convert ginsenosides into rare ginsenoside F 2 , compound K, compound O, compound Y, compound Mc1, compound Mc, C‑Mx1, and C‑Mx. Established a new method of using green, efficient, safe and edible edible and medicinal fungi to biotransform ginsenosides, which overcomes the shortcomings of using unsafe bacteria and molds that cannot be used in food and drug production as fermentation strains in the past .

Description

Technical field[0001]The invention relates to a preparation method of rare ginsenosides, discloses a method for preparing rare ginsenosides by using Schizophyllum biotransformation of ginsenosides, and belongs to the technical field of liquid fermentation of edible and medicinal fungi.Background technique[0002]Ginsenosides are the main effective components of the medicinal plants of the genus Panax ginseng, American ginseng and Panax notoginseng. It has the main physiological activities of plants of the genus Panax. So far, more than 100 kinds of ginseng monomer saponins have been isolated and determined. Ginsenosides are divided into glycol type ginsenosides (Rb1, Rc, Rb2, Rd, Rg3, Rh2, Compound K, etc.) and triol ginsenosides (Re, Rg1, Rg2, Rf, Rh1Etc.), in which ginsenoside Rb1, Rc, Rb2, Rd, Re and Rg1It is the main saponins of plants of the genus Ginseng, accounting for about 90% of ginsenosides. Rare ginsenosides, such as ginsenoside Rg3, F2, compound K, Rh2, Rh3 And so on, the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/20C12R1/645
CPCC12P33/20
Inventor 刘志李玉孙光芝阮长春王立娟
Owner JILIN AGRICULTURAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com