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Sturgeon bone gelatine iron chelating peptide and preparation method thereof

A technology of fish bone gelatin and gelatin iron, which is applied to the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve the problems that gelatin and metal chelating peptide technologies have not been reported, and achieve the effect of improving the utilization value

Active Publication Date: 2018-03-06
ZHEJIANG OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] After searching, there is no report on the technology of preparing gelatin and metal chelating peptides from sturgeon fish bones

Method used

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  • Sturgeon bone gelatine iron chelating peptide and preparation method thereof
  • Sturgeon bone gelatine iron chelating peptide and preparation method thereof

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Embodiment 1

[0025] A preparation method of sturgeon fish bone gelatin iron chelating peptide, the preparation process is as follows: sturgeon fish bone "gelatin extraction" double enzyme enzymatic hydrolysis "enzymatic hydrolyzate" ultrafiltration" macroporous resin purification "affinity chromatography" RP-HPLC preparation of "iron-chelating peptides.

[0026] 1) Pretreatment of sturgeon bones: add 0.1 mol / L NaOH solution to the sturgeon bones according to the ratio of solid to liquid 1 g:10 mL, soak for 5 h at 4 °C to remove non-collagen; Wash with distilled water repeatedly to pH 7.0, drain, add 0.5 mol / L EDTA solution with pH 7.4 according to the ratio of solid to liquid 1 g: 8 mL, soak at 4°C for 4 days, replace the EDTA solution once a day, decalcify, dry, Pulverize to obtain decalcified fish bone meal;

[0027] 2) Preparation of sturgeon fish bone gelatin: Add decalcified fish bone powder into double distilled water according to the ratio of solid to liquid 1 g:10 mL, extract dyna...

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Abstract

The invention relates to a sturgeon bone gelatine iron chelating peptide and a preparation method thereof, and belongs to the technical field of deep processing of aquatic products. The iron chelatingpeptide is prepared by the following steps: using sturgeon bone as a raw material to prepare gelatin, utilizing trypsin and alkaline protease to perform enzymatic hydrolysis on the gelatin, separating and purifying to obtain the iron chelating peptide Asp-Gly-Pro-Lys-Gly-Pro-His-Gly-Lys-Asp-Gly-Arg(DGPKGPHGKDGR) by ultrafiltration, macroporous resin column chromatography, affinity chromatographyand reversed-phase high performance liquid chromatography, and detecting that the molecular weight is 1220.28 Da by ESI / MS. The iron chelating peptide provide by the invention has strong iron chelating ability and is used for preparing iron supplement medicines and health products.

Description

technical field [0001] The invention belongs to the technical field of deep processing of aquatic products, and in particular relates to a sturgeon bone gelatin iron chelating peptide and a preparation method thereof. Background technique [0002] Gelatin has homology with collagen, and is a kind of protein obtained by partial hydrolysis of collagen. However, due to the difference in processing technology, the molecular weight is generally 15,000-250,000 Da. At present, gelatin is favored by more and more consumers due to its unique efficacy in the application of medicine, health care products, food and cosmetics, etc., and has become the focus of attention of the food industry, health care product industry and daily chemical industry. [0003] Trace elements are closely related to human survival and health, and play a vital role in human life. Excessive, insufficient, unbalanced or deficient intake of trace elements will cause physiological abnormalities or diseases in var...

Claims

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Application Information

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IPC IPC(8): C07K14/78C07K1/36C07K1/34C07K1/22C07K1/20C07K1/16C12P21/06
CPCC07K14/78C12P21/06
Inventor 王斌杨帆陶静赵玉勤
Owner ZHEJIANG OCEAN UNIV
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