Vacuum blood collection tube for storing and transporting circulating nucleic acid sample

Abstract

The invention discloses a vacuum blood collection tube, particularly a vacuum blood collection tube for storing and transporting a circulating nucleic acid sample. The vacuum blood collection tube comprises an original tube, a rubber plug and a protective cap, wherein the original tube is vacuumized; the rubber plug seals the original tube; a composite additive is arranged inside the original tube; the composite additive is obtained by mixing a chelating agent, an enzyme inhibitor, an osmotic agent, an antioxidant, an apoptosis inhibitor, a cell membrane stabilizer and a buffer solution. The invention can enable a collected sample to keep a whole-blood initial state under a room-temperature condition for 7-14 days without deterioration, and also can ensure that circulating nucleic acids inplasma or serum of whole blood are not degraded and whole blood cells are free of apoptosis and rupture, so that the circulating nucleic acids existing in the plasma or the serum are prevented from being contaminated by genomic nucleic acids, and adverse effects of a cross-linking action of a formaldehyde slow release agent-based preservation solution on subsequent experiments are avoided.

Description

technical field [0001] The invention relates to a vacuum blood collection tube, in particular to a vacuum blood collection tube for storing and transporting circulating nucleic acid samples. Background technique [0002] Circulating nucleic acid is a nucleic acid that exists in whole blood plasma and serum. Circulating nucleic acid is also called extracellular nucleic acid. Circulating nucleic acid can be obtained through blood collection, so how to preserve and transport samples containing circulating nucleic acid is very realistic. Technical problems, for this reason, people have developed collection tubes for storing and transporting circulating nucleic acid samples, but the current collection tubes or corresponding vacuum blood collection tubes for storing and transporting circulating nucleic acid samples have obvious defects, mainly in the collection The preservation solution added in the tube is basically the preservation solution of formaldehyde releasing agent, such ...

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the defects of the prior art that lead to low extraction of circulating nucleic acid, and to invent a vacuum blood collection tube that does not affect the storage and transportation of circulating nucleic acid samples at room temperature

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