Vacuum blood collection tube for storing and transporting circulating nucleic acid sample

A technology for blood collection and nucleic acid samples, which is applied to biological material sampling methods, microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problem of low extraction of circulating nucleic acid and achieve the effect of avoiding cross-linking

Inactive Publication Date: 2018-03-06
付士明
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the defects of the prior art that lead to low extraction of circulating nucleic acid, and to invent a vacuum blood collection tube that does not affect the storage and transportation of circulating nucleic acid samples at room temperature

Method used

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  • Vacuum blood collection tube for storing and transporting circulating nucleic acid sample
  • Vacuum blood collection tube for storing and transporting circulating nucleic acid sample
  • Vacuum blood collection tube for storing and transporting circulating nucleic acid sample

Examples

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Embodiment Construction

[0040] Such as figure 1 As shown, the vacuum blood collection tube for storing and transporting circulating nucleic acid samples is composed of an original tube 1, a rubber plug 2 and a protective cap 3. The original tube 1 is evacuated, and the rubber plug 2 is sealed on the original tube 1. It is characterized in that: the original tube 1 is provided with a composite additive 4, and the composite additive 4 is formed by mixing a chelating agent, an enzyme inhibitor, an osmotic pressure agent, an antioxidant, an apoptosis inhibitor, a cell membrane stabilizer, and a buffer.

[0041] Such as figure 2 As shown, the original pipe 1 is provided with a bracket 5, the bracket 5 is provided with an inner pipe 6, the bottom of the inner pipe 6 is provided with a channel 7 communicating with the original pipe 1, and the inner pipe 6 is provided with a filter core 8 , the wall of the inner tube 6 above the filter element 8 is sprayed with a drug-loaded layer 9 of anticoagulant or coa...

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Abstract

The invention discloses a vacuum blood collection tube, particularly a vacuum blood collection tube for storing and transporting a circulating nucleic acid sample. The vacuum blood collection tube comprises an original tube, a rubber plug and a protective cap, wherein the original tube is vacuumized; the rubber plug seals the original tube; a composite additive is arranged inside the original tube; the composite additive is obtained by mixing a chelating agent, an enzyme inhibitor, an osmotic agent, an antioxidant, an apoptosis inhibitor, a cell membrane stabilizer and a buffer solution. The invention can enable a collected sample to keep a whole-blood initial state under a room-temperature condition for 7-14 days without deterioration, and also can ensure that circulating nucleic acids inplasma or serum of whole blood are not degraded and whole blood cells are free of apoptosis and rupture, so that the circulating nucleic acids existing in the plasma or the serum are prevented from being contaminated by genomic nucleic acids, and adverse effects of a cross-linking action of a formaldehyde slow release agent-based preservation solution on subsequent experiments are avoided.

Description

technical field [0001] The invention relates to a vacuum blood collection tube, in particular to a vacuum blood collection tube for storing and transporting circulating nucleic acid samples. Background technique [0002] Circulating nucleic acid is a nucleic acid that exists in whole blood plasma and serum. Circulating nucleic acid is also called extracellular nucleic acid. Circulating nucleic acid can be obtained through blood collection, so how to preserve and transport samples containing circulating nucleic acid is very realistic. Technical problems, for this reason, people have developed collection tubes for storing and transporting circulating nucleic acid samples, but the current collection tubes or corresponding vacuum blood collection tubes for storing and transporting circulating nucleic acid samples have obvious defects, mainly in the collection The preservation solution added in the tube is basically the preservation solution of formaldehyde releasing agent, such ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/26C12N15/10
CPCC12N15/1003C12Q2527/125
Inventor 付士明
Owner 付士明
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