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Strain culture medicine favorable for preventing bacterial contamination

A strain culture medium and culture medium technology, applied in the field of microbiology, can solve the problems of slow proliferation of Streptococcus pneumoniae and easy occurrence of bacterial contamination, so as to achieve the effect of not easily contaminating bacteria and promoting the proliferation rate

Inactive Publication Date: 2018-03-06
CHENGDU OLYMVAX BIOPHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is that in the existing medium for cultivating Streptococcus pneumoniae, the proliferation rate of Streptococcus pneumoniae is relatively slow, and the phenomenon of bacterial contamination is prone to occur. The proliferation rate of Streptococcus pneumoniae is significantly improved, and the problem of bacterial infection is not easy to occur

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The invention provides a strain culture medium beneficial to anti-contamination bacteria, and the raw material composition of the culture medium is as follows in parts by weight:

[0017] Jerusalem artichoke, 15g, Codonopsis pilosula, 10g, wolfberry leaves, 10g; peptone, 20g, corn steep liquor, 15g, bran, 10g; MgSO 4 , 0.25g / L; NaCl, 2.0g / L; K 2 HPO 4 , 0.8g / L; ZnSO 4 , 0.1g / L; FeSO 4 , 0.01g / L; Niacin: 0.2g / L; Adenine: 3.5g / L; Cysteine, 0.8g / L; Agar, 15g; Water, 1000ml. The medium was adjusted to pH 6.8.

Embodiment 2

[0019] The present invention provides a kind of strain culture medium that is beneficial to anti-infection bacteria, and the composition ratio of the raw material of the culture medium is as follows according to weight g:

[0020] Jerusalem artichoke, 45g, Codonopsis pilosula, 30g, wolfberry leaves, 30g; peptone, 80g, corn steep liquor, 55g, bran, 35g; MgSO 4 , 2.0g / L; NaCl, 12.0g / L; K 2 HPO 4 , 3.0g / L; ZnSO 4 , 0.3g / L; FeSO 4 , 0.08g / L; Niacin: 1.2g / L; Adenine: 8.5g / L; Cysteine, 2.0g / L; Agar, 20g; Water, 1000ml. The medium was adjusted to pH 6.9.

Embodiment 3

[0022] The present invention provides a kind of strain culture medium that is beneficial to anti-infection bacteria, and the composition ratio of the raw material of the culture medium is as follows according to weight g:

[0023] Jerusalem artichoke, 25g, Codonopsis pilosula, 15g, wolfberry leaves, 15g; peptone, 40g, corn steep liquor, 25g, bran, 15g; MgSO 4 , 0.65g / L; NaCl, 5.0g / L; K 2 HPO 4 , 1.5g / L; ZnSO 4 , 0.18g / L; FeSO 4 , 0.03g / L; Niacin: 0.6g / L; Adenine: 5.5g / L; Cysteine, 1.2g / L; Agar, 15g; Water, 1000ml. The medium was adjusted to pH 7.0.

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PUM

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Abstract

The invention discloses a strain culture medicine favorable for preventing bacterial contamination. The culture medium comprises the following raw materials in parts by weight: 15 to 45 parts of jerusalem artichoke, 10 to 30 parts of codonopsis pilosula, 10 to 30 parts of medlar leaf, 20 to 80 parts of peptone, 15 to 55 parts of corn pulp, 10 to 35 parts of bran, 0.25 to 2.0 g / L of MgSO4, 2.0 to 12.0 g / L of NaCl, 0.8 to 3.0 g / L of K2HPO4, 0.1 to 0.3 g / L of ZnSO4, 0.01 to 0.08 g / L of FeSO4, 0.2 to 1.2 g / L of nicotinic acid, 3.5 to 8.5 g / L of adenine, 0.8 to 2.0 g / L of cysteine, 15 to 20 parts of agar and 1000 parts of water. The culture medium promotes obvious increment of proliferation rate of streptococcus pneumonia and is not liable to bacterial contamination.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a strain culture medium beneficial to anti-contamination bacteria. Background technique [0002] Streptococcus pneumoniae was first isolated from patient sputum in France and the United States by Louis Pasteur and G.M.Sternberg in 1881, respectively. It is Gram-positive, and the bacteria are like spearheads, diplococci arranged in pairs or in short chains, and some virulent strains have a capsule with a chemical composition of polysaccharides. 5% to 10% of normal people carry this bacterium in the upper respiratory tract, and the virulent strain is an important pathogenic bacterium that causes human diseases. The bacterial strain has certain applications in the field of biotechnology, but the growth rate of Streptococcus pneumoniae in the commonly used culture medium is relatively slow, and at the same time, bacterial contamination is prone to occur. Contents of the invention...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/46
CPCC12N1/20
Inventor 杜洪波王宇范杨艳李靖
Owner CHENGDU OLYMVAX BIOPHARM
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