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Extraction method and application of grub polysaccharide

An extraction method, grub technology, applied in the field of polysaccharide extraction, can solve the problems of extraction efficiency impact, polysaccharide degreasing, etc., and achieve the effect of reducing blood fat and health care, low price, and no toxic and side effects

Inactive Publication Date: 2018-03-13
湖北聚注通用技术研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Regarding the extraction of grub polysaccharides, Chinese patent CN201610838537.4 mentions a grub polysaccharide component and its preparation method and use, but this patent does not degrease the extracted polysaccharides, which affects the extraction efficiency

Method used

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  • Extraction method and application of grub polysaccharide
  • Extraction method and application of grub polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for extracting polysaccharides from grubs, comprising the steps of:

[0026] (1) Wash the grub, dry and pulverize it at 60°C, and pass through a 30-mesh sieve to obtain the grub dry powder;

[0027] (2) Take the grub dry powder in step (1), add petroleum ether to degrease, the mass volume ratio (g / mL) of the grub dry powder to petroleum ether is 1:6.5, dry after degreasing, obtain the defatted grub dry powder;

[0028] (3) Take the degreased grub dry powder of step (2), add 0.02mol / L NaOH solution, the mass volume ratio (g / mL) of defatted grub dry powder and NaOH solution is 1:13, 70 ℃ of water bath stirring and extracting 3 times, each time 2h, after filtering, combine the filtrate, adjust the pH of the filtrate to neutral, and concentrate under reduced pressure to 1 / 7.5 of the original volume;

[0029] (4) Take the concentrated solution of step (3), add 4 times the volume of 95% ethanol, put it in the refrigerator overnight, centrifuge, collect the precipita...

Embodiment 2

[0032] A method for extracting polysaccharides from grubs, comprising the steps of:

[0033] (1) Wash the grub, dry and pulverize it at 60°C, and pass through a 20-mesh sieve to obtain the grub dry powder;

[0034] (2) Take the grub dry powder in step (1), add petroleum ether to degrease, the mass volume ratio (g / mL) of the grub dry powder to petroleum ether is 1:10, dry after degreasing, obtain the defatted grub dry powder;

[0035] (3) Take the degreased grub dry powder of step (2), add 0.02mol / L NaOH solution, the mass volume ratio (g / mL) of defatted grub dry powder and NaOH solution is 1:20, 90 ℃ of water baths are stirred and extracted 3 times, each time 1h, after filtering, combine the filtrate, adjust the pH of the filtrate to neutral, and concentrate under reduced pressure to 1 / 5 of the original volume;

[0036] (4) Take the concentrated solution of step (3), add 4 times the volume of 95% ethanol, put it in the refrigerator overnight, centrifuge, collect the precipita...

Embodiment 3

[0039] A method for extracting polysaccharides from grubs, comprising the steps of:

[0040] (1) Wash the grub, dry and pulverize it at 60°C, and pass through a 40-mesh sieve to obtain the grub dry powder;

[0041] (2) Take the grub dry powder in step (1), add petroleum ether to degrease, the mass volume ratio (g / mL) of the grub dry powder to petroleum ether is 1:3, dry after degreasing, and obtain the degreased grub dry powder;

[0042] (3) Take the degreased grub dry powder in step (2), add 0.02mol / L NaOH solution, the mass volume ratio (g / mL) of defatted grub dry powder to NaOH solution is 1:5, stir and extract in a 50°C water bath for 3 times, each time 3h, after filtering, combine the filtrate, adjust the pH of the filtrate to neutral, and concentrate under reduced pressure to 1 / 10 of the original volume;

[0043] (4) Take the concentrated solution of step (3), add 4 times the volume of 95% ethanol, put it in the refrigerator overnight, centrifuge, collect the precipitat...

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Abstract

The invention discloses an extraction method and application of grub polysaccharide, and provides a pet health care product. The extraction method of the grub polysaccharide mainly comprises the stepsof washing and drying fresh grub, degreasing and drying, extracting alkali, precipitating alcohol, and finally separating and drying after deproteinizing to obtain the required grub polysaccharide. According to the extraction method and the application of the grub polysaccharide provided by the invention, during a grub polysaccharide extraction process, treatments such as degreasing and deproteinizing are carried out, so that the extraction rate and the quality of the grub polysaccharide can be effectively improved; the grub polysaccharide can be further added into the pet health care product, so that an effect on reducing blood fat is achieved.

Description

technical field [0001] The invention relates to a method for extracting polysaccharides, in particular to a method for extracting polysaccharides from grubs. Background technique: [0002] Grubs, also known as cockroaches, soil silkworms, old mother worms, white time worms, etc., are fat in body and are commonly called scarab beetles or scarab beetles when they become adults. Due to their unique protein and fat, most of them are directly ground into powder and used as feed and other high-protein foods. or high-fat uses, but little attention has been paid to insect polysaccharides, which are equally abundant in grubs. [0003] The Sevag method is an effective method for removing free protein from crude polysaccharides. Adding a certain amount of specific substances to the extracted crude polysaccharide can effectively promote the denaturation of free proteins into insoluble substances, and then remove these free proteins by centrifugation, which is safe and efficient without...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A23K10/30A23K20/163A23K50/40
CPCC08B37/0003A23K10/30A23K20/163A23K50/40
Inventor 不公告发明人
Owner 湖北聚注通用技术研究有限公司
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