Enoki mushroom mycelium pellet preservation method

Abstract

The invention relates to the technical field of strain preservation, in particular to an enoki mushroom mycelium pellet preservation method. The method includes steps: (1) material preparation, to bemore specific, respectively preparing a slant culture medium, a fluid medium and preservative fluid; (2) slant strain preparation, to be more specific, performing inoculation of an enoki mushroom strain into the slant culture medium prepared in the step (1) to obtain a slant strain; (3) enoki mushroom mycelium pellet preparation, to be more specific, performing inoculation of the slant strain obtained in the step (2) into the fluid medium prepared in the step (1) to obtain enoki mushroom mycelium pellets; (4) preservation, to be more specific, performing inoculation of the enoki mushroom mycelium pellets obtained in the step (3) into the preservative fluid prepared in the step (1), and preserving at a temperature of 0-4 DEG C. Providing nutrients for the enoki mushroom mycelium pellets ina preservation process is avoided, growth of the enoki mushroom mycelium pellets is greatly decelerated due to low preservation temperature, and the enoki mushroom mycelium pellets keep strain physiological activity and cellular morphology.

Description

technical field [0001] The invention relates to the technical field of preservation of strains, in particular to a method for preservation of Flammulina velutipes mycelium balls. Background technique [0002] At present, the strain preservation method of Flammulina velutipes has inclined-plane cryopreservation method, wheat grain preservation method, inclined-plane normal saline preservation method, and paraffin-sealing inclined-plane cryopreservation method (this method is to seal the test tube mouth with paraffin on the basis of inclined-plane cryopreservation method, to be paraffin solidification), bran preservation method, etc.; slope cryopreservation method and paraffin sealing preservation method have a short storage period, usually about half a year, and the time is long, the medium is dry and solid, and the mycelium turns yellow and aging; the production process of wheat grain preservation method It is complicated, the soaking time of wheat grains is difficult to gra...

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Problems solved by technology

[0002] At present, the strain preservation method of Flammulina velutipes has inclined-plane cryopreservation method, wheat grain preservation method, inclined-plane normal saline preservation method, and paraffin-sealing inclined-plane cryopreservation method (this method is to seal the test tube mouth with paraffin on the basis of inclined-plane cryopreservation method, to be paraffin solidification), bran preservation method, etc.; slope cryopreservation method and paraffin sealing preservation method have a short storage period, usually about half a year, and the time is long, the medium is dry and solid, and the mycelium turns yellow and aging; the production process of wheat grain preservation method It is complicated, the soaking time of wheat grains is difficult to control, and powder spores are easy to be produced; the operation of slant physiological saline preservation is simple, but the slant surface is easy to fall off after a long storage time, and the culture medium on the slant surface floats out of the water surface, causing the exposed part to dry and solidify, and the strains are aging, which is easy Variation, easy to produce powder spores during activation, affecting mushroom yield and quality

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