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A tumor-targeting photothermal agent, its preparation method and application

A tumor-targeting, photothermal technology, applied in the field of nanomaterials, to achieve the effects of inducing apoptosis, strong absorption, and high photothermal conversion efficiency

Active Publication Date: 2020-09-08
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

MoTe has not been seen so far 2 In Multifunctional Therapies for Cancer

Method used

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  • A tumor-targeting photothermal agent, its preparation method and application
  • A tumor-targeting photothermal agent, its preparation method and application
  • A tumor-targeting photothermal agent, its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] [Example 1] Synthesis of tumor-targeting photothermal agent 1:

[0025] (1) Molybdenum telluride crystals were milled in a high-speed ball mill for 48 h at a speed of 400 rpm.

[0026] (2) Use a cell pulverizer to evenly disperse the milled powder in deionized water. The pulverization time is 12 h and the temperature is 0-4 o c.

[0027] (3) Molybdenum telluride dispersed in water and HS-PEG-cRGD (Mn = 25.7 kg / mol) were mixed and stirred for 48 h at a mass ratio of 1:1, and then centrifuged at 8000 rpm to obtain the biophase MoTe with excellent capacitance 2 nanoflakes.

[0028] (4) Characterize the material structure by transmission electron microscopy ( figure 1 ), and the obtained product was stored at -18°C in the dark.

Embodiment 2

[0029] [Example 2] UV-Vis absorption spectrum of tumor-targeting photothermal agent 1

[0030] MoTe 2 The UV-Vis absorption spectra of aqueous nanoflakes were measured by a UV-Vis spectrophotometer (ThermoScientific, Waltham, MA). The result is as figure 2 As shown, MoTe 2 The UV-vis absorption spectrum of nanoflakes shows strong near-infrared absorption at 800–950 nm, thus proving that MoTe 2 Nanoflakes are a compound with photothermal conversion potential, and the optimal absorption wavelength range is 800-950 nm.

Embodiment 3

[0031] [Example 3] Tumor-targeted photothermal agent 1 acts on mouse breast cancer cells

[0032] Mouse breast cancer cells were seeded in two 96-well plates at a density of 6000 cells / well, and cultured with 0.1 ml of medium for 24 hours. Then, the medium was prepared into different concentrations of MoTe 2 The PBS solution of nanoflakes was added to each well respectively. Incubate at 37°C for 48 hours under dark conditions. The dark toxicity group continued to culture for 24 hours under the condition of avoiding light. To assess the phototoxicity of the material, the material that had not been endocytosed was replaced with fresh medium after 6 hours of culture, and then irradiated with a NIR laser. Near-infrared light at 808 nm with a power of 1 W cm per well -1 Laser irradiation for 1 min. The incubation was continued for another 24 hours. Then, add 20 µl of MTT (MTT dissolved in 5 mg / ml PBS buffer) to each well. After co-cultivation for 4 hours, the medium was aspi...

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Abstract

The invention discloses a targeting photothermal medicament for a tumor, a preparation method and application. According to the preparation method, the hydrophilic modification is carried out on molybdenum telluride through sulfydryl modified PEG (Polyethylene Glycol) with a targeting group; the medicament with a photothermal anti-tumor effect is synthesized. The medicament has the characteristicof targeting a tumor cell and moreover, the curative effect of inhibiting the proliferation of the tumor cell can be realized through a photothermal effect.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials, and in particular relates to a tumor-targeting photothermal agent, its preparation method and application. Background technique [0002] Photothermal therapy is an emerging treatment method based on far-infrared waves generated by light-excited materials. With the development of photothermal reagents such as conjugated organic groups, carbon nanotubes, and gold nanorods, photothermal therapy has been widely used in tumor imaging and treatment. The specificity and selectivity of photothermal agents play a key role in the process of diagnosis and treatment. The geometric structure of nanomaterials has a great influence on their photothermal effect. In recent years, MoS with nanoscale thickness 2 , MoSe 2 , W 2 , WSe 2 Transition metal dichalcogenides have attracted extensive attention. Due to their unique physical and chemical properties, transition metal dichalcogenides have strong ap...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K9/51A61P35/00
CPCA61K9/5146A61K41/0052
Inventor 张先正马宁张明康曾旋
Owner WUHAN UNIV
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