Method for constructing lung cancer multi-gene mutation library
A construction method and library technology, applied in the field of construction of lung cancer multi-gene mutation library, can solve the problems of low sensitivity, large sample size requirements, and high cost
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[0256] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.
[0257] For plasma samples, genomic DNA / RNA was extracted using the plasma DNA / RNA extraction kit from Qiagen, and the specific steps were according to the instructions of the kit. No less than 1000 μl of plasma was extracted each time. The extracted DNA / RNA was dissolved in Tris-HCl (10mmol / L, pH 8.0), the quality of the extraction was detected by a UV spectrophotometer, and the concentration was determined, and the DNA / RNA was adjusted with Tris-HCl solution (10mmol / L, pH 8.0). Concentration to 2ng / μl as a template for PCR amplification.
[0258] The kit based on the above construction method includes:
[0259] A DNA enrichment reaction component, consisting of the first amplification primer set, the recipe of the DNA enrichment reaction component per person is shown in the table below:
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