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Preparation method of whey protein hydrolyzate

A technology of whey protein hydrolysis and whey protein powder is applied in the field of preparing whey protein hydrolyzate, which can solve the problems of insufficient hydrolysis, low efficiency, easy generation of bitter peptides and the like

Active Publication Date: 2021-09-07
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The inventors found that the current method of hydrolyzing whey protein has problems such as low efficiency, insufficient hydrolysis, difficulty in absorption by the human body, and easy production of bitter peptides.

Method used

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  • Preparation method of whey protein hydrolyzate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1) Mix whey protein powder WPC30 and WPC80 according to the mass ratio of 3:1, and the total mass of the two is 180g;

[0035] 2) 800mL of purified water, heat up to 50°C, add the whey powder mixed in step 1), keep stirring for 30min, then let stand for 30min to fully hydrate;

[0036] 3) The whey powder solution after hydration is sterilized for 2 minutes at a temperature of 105°C;

[0037] 4) Add 3 g of phosphate to the solution obtained in step 3) to adjust the pH to 5.5;

[0038] 5) The neutral protease derived from Aspergillus oryzae and Aspergillus flavus is prepared in advance as a solution, the concentration is 0.05%, and 0.1g (0.01%) calcium chloride is added as an activator;

[0039] 6) Add 200 ml of the 0.05% neutral protease solution obtained in step 5) to the solution obtained in step 4), stir slowly, and digest for 7 to 9 hours while maintaining the temperature at 35° C. to 40° C.;

[0040] 7) The digested product is subjected to enzyme inactivation trea...

Embodiment 2

[0042] 1) Mix the desalted whey powder and concentrated whey protein according to the ratio of 1:6, and the total mass of the two is 150g;

[0043] 2) Heat 780mL of purified water to 55°C, add the whey powder mixed in step 1), keep stirring for 15 minutes, and then let it stand for 40 minutes to fully hydrate;

[0044] 3) The whey powder solution after hydration is sterilized for 1 min at a temperature of 115°C;

[0045] 4) Add 0.5 g of phosphate to the solution obtained in step 3) to adjust the pH to 8.5.

[0046] 5) The neutral protease derived from Aspergillus oryzae was prepared as a solution in advance, with a concentration of 1.3%, and 0.2 g of sodium ferric edetate was added as an activator;

[0047] 6) Add 60 mL of the 1.3% neutral protease solution obtained in step 5) to the solution obtained in step 4), stir slowly, and digest at a temperature of 55° C. to 60° C. for 3 to 4 hours;

[0048] 7) The digested product is subjected to an enzyme inactivation treatment at ...

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Abstract

The present invention proposes a method for preparing a whey protein hydrolyzate, the method comprising: using peptidase to digest whey protein, so as to obtain the whey protein hydrolyzate; wherein, the peptidase is an aqueous solution of peptidase The concentration is 0.01%-1.5%; the digestion treatment is carried out at a temperature of 35°C-60°C for 2-10 hours. The method has higher efficiency in preparing whey protein hydrolyzate, can reduce the allergenicity of whey protein, increases the content of polypeptides and amino acids in whey protein hydrolyzate, and the obtained whey protein hydrolyzate can achieve rapid absorption and ease movement Fatigue and other functional effects, and no bitter peptides are produced, the taste is better.

Description

technical field [0001] The invention relates to the field of food, in particular, the invention relates to a method for preparing whey protein hydrolyzate. Background technique [0002] Whey protein is a kind of protein extracted from milk. It has the characteristics of high nutritional value, easy digestion and absorption, and contains a variety of active ingredients. It is known as the king of protein and is recognized as one of the high-quality protein supplements for human body. Whey protein is a high-quality complete protein. It is convenient to take, has a high absorption utilization rate, and can reduce the burden on the stomach. It is the first choice for human body to supplement protein. [0003] Whey protein refers to the general term for the remaining proteins in the whey after the casein is precipitated at a pH of 4.6, accounting for about 20% of the total milk protein, mainly including β-lactoglobulin, α-lactalbumin, serum albumin, and immunoglobulin. Whey prot...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 康正雄李树森李辉白者米来李洪亮
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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