Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for targeted step-by-step regulation and control of membrane permeability of microcapsule based on molecular imprint strategy

A molecular imprinting and microcapsule technology, which is applied in the analysis of materials, material excitation analysis, and material analysis by optical means, can solve the problems of difficult and precise targeting adjustment, single discontinuous adjustment process, etc. The effect of adjustment

Inactive Publication Date: 2018-03-30
HARBIN INST OF TECH
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that the adjustment process of the existing membrane permeability adjustment method is single and discontinuous and it is difficult to precisely target adjustment, and to provide a method based on molecular imprinting strategy to target and adjust the membrane permeability of microcapsules step by step

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for targeted step-by-step regulation and control of membrane permeability of microcapsule based on molecular imprint strategy
  • Method for targeted step-by-step regulation and control of membrane permeability of microcapsule based on molecular imprint strategy
  • Method for targeted step-by-step regulation and control of membrane permeability of microcapsule based on molecular imprint strategy

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0026] Specific implementation mode 1: This implementation mode records a method based on the molecular imprinting strategy to target and regulate the membrane permeability of microcapsules step by step. The specific steps of the method are as follows:

[0027] Step 1: Preparation of dextran-polymer coupling body: 1.00 g dextran and 1.85 g succinic anhydride were fully dissolved in 5 mL dimethyl sulfoxide (DMSO), and dissolved in 0.05 g 4-dimethylaminopyridine (DMAP ) under the catalysis of 60 ℃ for 12~16 h to obtain a transparent liquid, the transparent liquid and deionized water according to the volume ratio of 1:50, add deionized water to the transparent liquid, use a 3kDa dialysis bag Dialyze for 48~72 h, and then dry with a freeze dryer to obtain carboxylated dextran (Dex-COOH); weigh 3.50 g of 1,6-ethylenediamine and adjust the pH to 5.5~6.5 with hydrochloric acid, then add 1,6-ethanediamine The diamine solution was slowly added dropwise to 5 mL of an aqueous solution co...

specific Embodiment approach 2

[0043] Embodiment 2: In Embodiment 1, a method for targeted step-by-step regulation of microcapsule membrane permeability based on molecular imprinting strategy, in step 2, the molecular weight of the lysozyme is 14 kDa.

specific Embodiment approach 3

[0044] Embodiment 3: In Embodiment 1, a molecular imprinting strategy-based method for step-by-step regulation of microcapsule membrane permeability, in step 3, the molecular weight of bovine serum albumin is 67 kDa.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
molecular weightaaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to a method for targeted step-by-step regulation and control of membrane permeability of a microcapsule based on a molecular imprint strategy, and belongs to the technical fieldof membrane permeability regulation and control. The method comprises the following steps: 1, preparing a dextran-polymer coupled body element; 2, preparing a lysozyme-polymer coupled body element; 3,preparing a bovine serum albumin-polymer coupled body element; 4, constructing a ternary hybrid microcapsule; and 5, using a molecular imprinting strategy to regulate and control the membrane permeability of the microcapsule step by step. The method has the following advantages: a protein capsule and a molecular imprinting strategy are combined to construct a ternary hybrid biological microcapsule, and then different elements are imprinted and etched step by step according to a sequence from small to large of molecular weights under different conditions to finally form pore channels with different sizes, so that a simple and effective step-by-step regulation and control method for the surface membrane permeability the microcapsule is realized, and the problem that the membrane permeability of the microcapsule is difficult to accurately target and regulate is solved.

Description

technical field [0001] The invention belongs to the technical field of membrane permeability regulation, and in particular relates to a method for targeted step-by-step regulation of microcapsule membrane permeability based on a molecular imprinting strategy. Background technique [0002] Microcapsules have been gradually developed in various important fields as protocell models, drug carriers, bioreactors and other functional models. Generally speaking, a microcapsule is a closed microcompartment composed of a membrane structure with a special structure and function. As a special barrier between the contents of the microcapsule and the external environment, the membrane structure must be able to make itself independent of the environment. , and can carry out selective material exchange activities or drug release processes with the surrounding environment through its membrane permeability. The permeability of the membrane directly determines what kind of functional model the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N21/6458G01N2021/6439
Inventor 刘小曼刘丽娜苏东悦王磊黄鑫
Owner HARBIN INST OF TECH