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Living body single-cell in-situ lysis and online ionization detection mass spectrum interface apparatus

A technology of ionization detection and interface device, which is applied in the field of single-cell mass spectrometry research, can solve the problems of low loading rate of inkjet printing technology, differences between cells and adherent states, and inability to separate media, and achieve real-time monitoring of metabolic levels, Avoid the interference of components, and the effect of fast cell processing speed

Active Publication Date: 2018-03-30
TSINGHUA UNIV
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  • Abstract
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Problems solved by technology

The probe penetration technology can only obtain a part of the sample in the cell, and cannot truly reflect the level of the cell to the corresponding substance
Inkjet printing technology also has problems such as low loading rate, inability to separate from the medium, and huge differences between cells and adherent states.

Method used

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  • Living body single-cell in-situ lysis and online ionization detection mass spectrum interface apparatus
  • Living body single-cell in-situ lysis and online ionization detection mass spectrum interface apparatus
  • Living body single-cell in-situ lysis and online ionization detection mass spectrum interface apparatus

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Embodiment Construction

[0017] In describing the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "vertical", "horizontal", "top", "bottom", "inner", " The orientation or positional relationship indicated by "outside", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the referred device or element must have a specific orientation, so as to Specific orientation configurations and operations, therefore, are not to be construed as limitations on the invention. In addition, the terms "first", "second", etc. are used for descriptive purposes only, and should not be construed as indicating or implying relative importance. The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0018] Such as figure 1 As shown, the present inve...

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Abstract

The invention relates to a living body single-cell in-situ lysis and online ionization detection mass spectrum interface apparatus. The apparatus comprises a cell control platform, a probe alignment apparatus, a micro fluid probe, an online ionization apparatus and a detection apparatus; the micro fluid probe and the online ionization apparatus are arranged on the cell control platform and the probe alignment apparatus; a cell sample is subjected to in-situ lysis in the cell control platform and the probe alignment apparatus; and then the processed cell sample passes through the micro fluid probe and the online ionization apparatus to form ionized mist spray to enter the detection apparatus. The detection apparatus disclosed in the invention integrates cell sample automatic control, cell in-situ lysis, lysis product automatic transport, mist spray driven liquid flow transport and mass spectrometric detection into an integrated body, so that lysis, lysis product transport and online mass spectrometric detection of any living body single cell can be performed.

Description

technical field [0001] The invention relates to the technical field of single-cell mass spectrometry research, in particular to an in-situ lysing and online ionization detection mass spectrometry interface device for living single cells. Background technique [0002] Single-cell detection is the analysis of cell content, metabolites, and genome at the single-cell level by means of mass spectrometry, capillary electrophoresis, and liquid chromatography. With the advancement of technology, scientists have developed more and more single-cell detection techniques. Single-cell analysis can provide important information for disease detection, gene sequencing, and cell metabolomics, so it has attracted wide attention. Single-cell detection has played an increasingly important role in drug development, disease prevention, and cell analysis mechanism and cell pathway research. [0003] There are many kinds of instruments for cell analysis, including optical imaging, fluorescence an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01J49/02H01J49/04G01N30/72
CPCG01N30/72H01J49/02H01J49/0404
Inventor 林金明毛思锋黄秋实
Owner TSINGHUA UNIV
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