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Special oscillation type sperm collector for sea urchin and use method thereof

A technology for extracting sperm and sea urchin, which is applied in the field of sea urchin reproduction research, can solve the problems that cannot be separated from manual operation procedures, the purity of sperm eggs is not high, and the quality is not good, so as to improve the collection efficiency, improve the quality of sperm, and use simple methods. Effect

Active Publication Date: 2018-04-03
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] All the methods mentioned above have disadvantages such as inability to break away from the manual operation procedure, time-consuming and labor-intensive, or low purity and poor quality of the extracted sperm and eggs.

Method used

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  • Special oscillation type sperm collector for sea urchin and use method thereof
  • Special oscillation type sperm collector for sea urchin and use method thereof
  • Special oscillation type sperm collector for sea urchin and use method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Such as figure 1 As shown, the special oscillating sperm extractor for sea urchins includes an electric oscillating cylinder main body 1, one side of the electric oscillating cylinder main body 1 is connected with a sperm preservation liquid release mechanism 2 through a bracket I11, and the other side of the electric oscillating cylinder main body 1 is connected through a bracket II16 is connected with an electric semen absorption mechanism 3, which is provided with an absorption hose 13 for absorbing semen and a semen storage container 14 for storing semen, and one end of the absorption hose 13 extends to the inside of the main body 1 of the electric vibration cylinder, The other end of the absorption hose 13 extends to the bottom of the semen container 14 . The main body 1 of the electric vibrating cylinder is used to place sea urchins and make the sea urchins vibrate at low frequency to ensure the normal discharge of semen. The sperm preservation solution release m...

Embodiment 2

[0041] Such as Figure 2~6 As shown, on the basis of Embodiment 1, this embodiment is further optimized as follows: the first end of the elastic fixing sheet 6 is connected with a pressing tube 17 with an opening downward, and the pressing tube 17 is surrounded by a tube wall 17b to form a tube inner space 17a, and the tube wall The thickness ratio H1:H2 of both ends of 17b is 1:2.2~3.1. The pressing tube 17 connected to the surface of the elastic fixing sheet 6 can be used to place the spines on the surface of the sea urchin to strengthen the fixing of the sea urchin, and the two ends of the tube wall 17b are provided with a thickness ratio so that the space in the tube 17a has a slope that fits the spines, The damage to the sea urchin is effectively avoided when the sea urchin is fixed, the protection of the sperm extractor to animals is improved, and the simultaneous development of economy and ecology is realized.

[0042] One end of the absorption hose 13 extends to the i...

Embodiment 3

[0046] Such as Figure 2~6 As shown, the sea urchin special vibration type sperm extractor, in the present embodiment, the optimal parameters of the sperm extractor are set as follows:

[0047] 1) The power of the motor 8 is 30w, and the oscillation frequency is 200 times / min;

[0048] 2) Electric suction air pump 15 generates a negative pressure of 50kpa;

[0049] 3) The diameter of the electric vibration support 5 tray is 3cm;

[0050] 4) The thickness ratio H1:H2 at both ends of the tube wall 17b of the pressing tube 17 is preferably 1:2.5;

[0051] 5) The radius ratio r1:r2 of the large suction port 13a and the small suction port 13b of the absorption hose 13 is preferably 1:0.68;

[0052] 6) The ratio of the length of the partition 13c in the absorption hose 13 to the length of the absorption hose 13 is 1:26;

[0053] 7) The micro-current source 18 outputs a constant current of 3.5 μA, and the current output frequency is 0.1 Hz

[0054] The above optimal parameter se...

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Abstract

The invention discloses a special oscillation type sperm collector for sea urchin and a use method thereof, and belongs to the technical field of reproduction study of sea urchin. The special oscillation type sperm collector comprises an electrical oscillation cylinder body, wherein one side of the electrical oscillation cylinder body is connected with a sperm preservation liquid releasing mechanism through a bracket I, and the other side of the electrical oscillation cylinder body is connected with an electrical sperm absorbing mechanism through a bracket II. The special oscillation type sperm collector for the sea urchin and the use method thereof have the advantages that the problem of difficulty in avoiding of manual sperm collection of the sea urchin in the embryo development study issolved; the matured sperms of the sea urchin can be automatically and efficiently collected; an integration combined device integrating electrical oscillation and electrical suction is matched with aspecial sperm discharging stimulation reagent, so as to solve the problem, and realize the purpose of automatically and efficiently collecting the matured sperms of the sea urchin; the special oscillation type sperm collector and the use method thereof are applied to the embryology study of the sea urchin and the artificial breeding and production practice of the sea urchin.

Description

technical field [0001] The invention belongs to the technical field of sea urchin reproduction research, and in particular relates to a special vibration-type sperm extractor for sea urchins and a use method thereof. Background technique [0002] Sea urchins belong to the class Echinoidea of ​​the door Echinodermata. At present, there are more than 850 species of sea urchins that have been discovered in the world, and there are more than 100 species in my country. However, most sea urchins are not edible. Horse dung sea urchins, purple sea urchins, white spines three-row sea urchins, sea hedgehogs, etc. Because sea urchin has high edible value, medicinal value and economic value, its economic species has also become fishery resources in developed countries such as the United States and Japan since the 1950s, and has been used as one of the main breeding species in recent years. [0003] Therefore, sea urchin breeding activities are becoming more and more frequent in my coun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D19/02A61D7/00A61D3/00
CPCA61D3/00A61D7/00A61D19/02A61D19/021A61D19/022
Inventor 叶莹莹李继姬桂福坤
Owner ZHEJIANG OCEAN UNIV