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QC (quality control) method of nucleic acid extraction kit for plants as well as plant leaf grinding device

A technology for nucleic acid extraction reagents and plant leaves, which is applied in the field of molecular biology, can solve problems such as prolonging production progress and wasting production costs, and achieve the effects of shortening production cycles, ensuring uniformity, and saving production costs

Inactive Publication Date: 2018-04-03
YANCHENG TIANSHUN MACHINERY TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the plant samples to be extracted are selected separately, and in the QC process, according to the principle of single variable experiment, it is assumed that the selected plant samples are the same. Once an error occurs in the experiment, the first step is to look for unqualified samples from the perspective of reagents. product, and then re-do QC, if it is only because of experimental errors caused by differences in plant samples, re-doing QC will inevitably waste production costs and prolong the production schedule

Method used

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  • QC (quality control) method of nucleic acid extraction kit for plants as well as plant leaf grinding device
  • QC (quality control) method of nucleic acid extraction kit for plants as well as plant leaf grinding device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Such as figure 1 (wherein the box body 1 is a perspective view), a plant leaf grinding device includes a box body 1 capable of cooling, and the box body 1 successively includes a connected grinding sample area 2 and a weighing area 3, and the weighing area 3 is provided with a weighing device 4, and the bottom of the box body 1 located in the weighing area 3 is provided with several funnel-shaped sampling mechanisms 5, and a fixed plate 6 is provided below the sample output mechanism 5 and a mechanism for driving the fixed plate 6 to move up and down The transmission device 7 , the transmission device 7 includes a fixed shaft 71 and a cylinder 72 connected thereto, and one end of the fixed shaft 71 is fixedly connected with the fixed plate 6 . The cylinder 72 drives the fixed shaft 71 to move up and down, thereby driving the fixed plate 6 to move up and down. A centrifuge tube 61 is movable on the fixed plate 6. It can be that the fixed plate 6 is provided with a speci...

Embodiment 2

[0029] 1. Experimental samples

[0030] Select soybean leaves, including large leaves and young leaves as plant samples to carry out experiments; use the plant leaf grinding device of embodiment 1 to distribute the mixed and ground powder of equal amounts of large leaves and young leaves into 6 centrifuge tubes, each Each centrifuge tube was filled with 1g of powder as the experimental group; using conventional methods, the large leaves and young leaves were divided into six grinding times. After sampling and grinding, 1g of powder was weighed into 6 centrifuge tubes as the control group.

[0031] 2. Nucleic acid extraction kit

[0032] Choose a Sigma Plant Nucleic Acid Extraction Kit for a small amount of nucleic acid extraction.

[0033] 3. Perform the test operation according to the instructions of the nucleic acid extraction kit in 2. After the nucleic acid is extracted, measure the concentration and purity of the nucleic acid.

[0034] 4. Experimental results

[0035] ...

Embodiment 3

[0041] According to the experimental results of Example 2, the QC of a plant nucleic acid extraction kit can be carried out in the following manner, comprising the following steps:

[0042] S1: Determine the amount of plant sample required

[0043]Take the previously qualified nucleic acid extraction kit for plants as a set of control experiments, and replace each reagent in the newly configured nucleic acid extraction kit for plants with the same reagents in the previously qualified nucleic acid extraction kit for plants to do multiple experiments , as the experimental group;

[0044] S2: sample grinding

[0045] According to the amount of plant samples required in step S1, take a certain amount of plant leaves and grind them into powder in a low temperature environment, and then divide the obtained powder into centrifuge tubes to ensure that the quality of the sample powder in each centrifuge tube is the same ; The plant sample powders of the control group and the experime...

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Abstract

The invention provides a plant nucleic acid extraction kit QC method and a plant leaf grinding device, specifically relating to the technical field of molecular biology, after mixing and grinding plant samples, they are then divided into each centrifuge tube for nucleic acid extraction experiments, and the plant samples are mixed The grinding device includes a box that can be refrigerated, and the box includes a connected grinding sample area and a weighing area with a weighing device in turn. The bottom of the box located in the weighing area is provided with several A sample discharging mechanism, a fixed plate with centrifuge tubes and a transmission device that drives the fixed plate to move up and down are provided below the sample discharging mechanism, and the sample discharging end of the sample discharging mechanism is movably inserted into the centrifuge tube, The diameter of the sampling end of the sampling mechanism is larger than the diameter of the centrifuge tube. The invention can ensure the homogeneity of plant samples, avoid re-QC of the newly prepared plant nucleic acid extraction kit due to plant samples, thereby reducing production cost and production cycle.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a QC method of a plant nucleic acid extraction kit and a plant leaf grinding device. Background technique [0002] Nucleic acid is a biological macromolecular compound synthesized by many nucleotides, and is one of the most basic substances of life. Nucleic acid widely exists in all animal and plant cells and microorganisms. Nucleic acid in organisms is often combined with protein to form nucleoprotein. Different nucleic acids have different chemical compositions and nucleotide arrangement sequences. According to different chemical composition, nucleic acid can be divided into ribonucleic acid (referred to as RNA) and deoxyribonucleic acid (abbreviated as DNA). DNA is the main material basis for storing, replicating and transmitting genetic information. RNA plays an important role in the process of protein synthesis-transfer ribonucleic acid, referred to ...

Claims

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Application Information

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IPC IPC(8): C12N15/10C12M1/00C12M1/34
CPCC12N15/1003
Inventor 王建坤
Owner YANCHENG TIANSHUN MACHINERY TECH CO LTD