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Preparation method of active chlorella polysaccharide

A technology of chlorella and chlorella powder, which is applied in the field of natural active ingredients, can solve the problems of low extraction efficiency and low activity of chlorella polysaccharides, and achieve high extraction efficiency, simple and feasible extraction method, good application value and market potential effect

Inactive Publication Date: 2018-04-06
金华市铁骑士生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the extraction efficiency of this extraction method is low, and the obtained chlorella polysaccharide activity is lower

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A method for preparing active chlorella polysaccharides, comprising ultrasonic crushing, preparation of chlorella crude polysaccharides, enzymatic hydrolysis-Sevage method for protein removal, and polysaccharide modification. The specific steps are:

[0016] 1) Ultrasonic crushing: mix chlorella powder and water evenly according to the material-liquid ratio of 1:12, then add 8-chlorophylline, ultrasonically crush for 15 minutes under the condition of ultrasonic power of 500MPa, and then place it at a speed of 7000r / Centrifuge 15min in the centrifuge of min, leave supernatant, standby, 8-chlorophylline is 0.22‰ of the weight of defatted chlorella powder;

[0017] 2) Preparation of crude polysaccharide from Chlorella: adjust the pH of the supernatant obtained in the ultrasonic crushing step to 10.2, then extract at a temperature of 65°C for 5 hours, then centrifuge in a centrifuge at a speed of 3000r / min for 15min, and place The clear liquid is the chlorella crude polysa...

Embodiment 2

[0021] A method for preparing active chlorella polysaccharides, comprising ultrasonic crushing, preparation of chlorella crude polysaccharides, enzymatic hydrolysis-Sevage method for protein removal, and polysaccharide modification. The specific steps are:

[0022] 1) Ultrasonic crushing: mix chlorella powder and water evenly according to the material-liquid ratio of 1:8, then add 8-chlorotheophylline, ultrasonically crush for 8 minutes under the condition of ultrasonic power of 520MPa, and then place it at a speed of 9000r / Centrifuge 8min in the centrifuge of min, leave supernatant, standby, 8-chlorophylline is 0.33‰ of the weight of defatted chlorella powder;

[0023]2) Preparation of crude polysaccharides from Chlorella: adjust the pH of the supernatant obtained in the ultrasonic crushing step to 9.5, then extract at 75°C for 3 hours, then centrifuge in a centrifuge at a speed of 5000r / min for 8 minutes, and place on The clear liquid is the chlorella crude polysaccharide s...

Embodiment 3

[0027] A method for preparing active chlorella polysaccharides, comprising ultrasonic crushing, preparation of chlorella crude polysaccharides, enzymatic hydrolysis-Sevage method for protein removal, and polysaccharide modification. The specific steps are:

[0028] 1) Ultrasonic crushing: mix chlorella powder and water evenly according to the material-liquid ratio of 1:10, then add 8-chlorophylline, and ultrasonically crush for 12 minutes under the condition of ultrasonic power of 510MPa, and then place it at a speed of 8000r / Centrifuge 10min in the centrifuge of min, leave supernatant, standby, 8-chlorophylline is 0.28‰ of the weight of defatted chlorella powder;

[0029] 2) Preparation of crude polysaccharides from Chlorella: adjust the pH of the supernatant obtained in the ultrasonic crushing step to 10.0, then extract at a temperature of 70°C for 4 hours, and then centrifuge for 10 minutes in a centrifuge at a speed of 4000r / min, The clear liquid is the chlorella crude po...

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Abstract

The invention discloses a preparation method of active chlorella polysaccharide. The preparation method comprises specific steps as follows: chlorella powder and water are mixed uniformly, 8-chlorotheophylline is added, ultrasonic disruption and centrifugation are performed, and a liquid supernatant is left; the pH of the liquid supernatant is adjusted, centrifugation is performed after extraction, and the liquid supernatant is a crude polysaccharide solution of chlorella; protein is removed with an enzymolysis-Sevage method, the liquid supernatant is dried, and deproteinized polysaccharide isobtained; chlorella polysaccharide is dissolved in anhydrous formamide, stirring is performed in an ice water bath, an esterification reagent is added for a reaction, centrifugation is performed after reaction ends, a liquid supernatant is collected, dialysis, concentration, alcohol precipitation and centrifugation are performed, precipitates are mixed with water for re-dissolution, concentrationand freeze drying are performed, and the active chlorella polysaccharide is obtained. The preparation method has the benefits as follows: the extraction method is simple and feasible, the mechanicaloperation degree is high, the extraction efficiency is high, short time is consumed, the extraction rate of chlorella polysaccharide is high, the activity is high, industrial large-scale production iseasy, and the preparation method has good application value and market potential.

Description

technical field [0001] The invention relates to the field of natural active ingredients, in particular to a preparation method of active chlorella polysaccharides. Background technique [0002] The ocean covers the surface area of ​​the earth, and the vast ocean area and its special environment lead to the diversity of marine biological species far exceeding the diversity of terrestrial biological species. The types and resources of marine microorganisms are immeasurable and diverse. Compared with the terrestrial environment, due to the special living environment such as ocean high pressure, high salinity, low temperature, especially deep sea or local high temperature, oligotrophic, and no light, marine microorganisms not only survive in extreme ecological environments with their unique biological metabolism , and can also produce active metabolites different from terrestrial microorganisms. Special environmental microorganisms are biological resources with special functio...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 全盈园
Owner 金华市铁骑士生物科技有限公司
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