Moderate halophilic aromatic pollutant degrading bacteria and application thereof

A technology for degrading bacteria and aromatics, applied in the restoration of polluted soil, bacteria, microorganisms, etc., can solve the problems of poor removal effect of PAHs, achieve potential application value, reduce ecological toxicity, and high degradation rate

Pending Publication Date: 2018-04-06
EAST CHINA UNIV OF SCI & TECH
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it has been reported that many bacteria, fungi, and algae have the ability to degrade PAHs, but studies have found that the removal of PAHs is poor when the non-halophilic PAHs-

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Moderate halophilic aromatic pollutant degrading bacteria and application thereof
  • Moderate halophilic aromatic pollutant degrading bacteria and application thereof
  • Moderate halophilic aromatic pollutant degrading bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A moderately halophilic polycyclic aromatic hydrocarbon degrading bacterium, which was preserved in the China Type Culture Collection Center CCTCC on June 30, 2011, with the number CCTCC NO: M2011218, and the name is moderately halophilic polycyclic aromatic hydrocarbon degradation bacteria, Martelella sp. AD-3.

[0037]Pick a single colony of Martelella sp.AD-3 and inoculate it in a -1 Cultured in phenanthrene mineral medium for 2 days. 30mL of different concentrations of phenanthrene (100mg·L -1 , 200mg·L -1 , 300mg·L -1 , 400mg·L -1 ) culture medium were placed in 100mL Erlenmeyer flasks, according to 5% inoculum size (D 600 =0.05), at 30°C, 150r·min -1 Shake culture in the dark. Take samples at regular intervals and measure D 600 and the residue of phenanthrene, and respectively change the culture temperature, the concentration of phenanthrene, the initial pH value, the salt concentration of the medium, and study the influence of each factor on the degradati...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses moderately halophilic bacteria capable of efficiently degrading multiple polycyclic aromatic hydrocarbons and application thereof, wherein the moderately halophilic bacteria are enriched and screened from polycyclic aromatic hydrocarbon contaminated saline alkali soil in Shengli Oil Field, are identified and named as Martelella sp.AD-3(CCTCC M2011218). The bacteria can efficiently degrade multiple pollutants such as biphenyl, acenaphthylene, acenaphthene, fluorene, phenanthrene, anthracene, fluoranthene and benzo[a]anthracene, and chrysene under the condition that the salinity is 0.5-15% and the pH value is 6-10. In 36h, degradation rate for fluorene with concentration of 100mg/L can reach 100%. The optimal degradation conditions of AD-3 bacteria are that pH is 9.0,salinity is 15g/kg, moisture content is 25%, and temperature 30 DEG C. Under the optimal conditions, AD-3 bacteria have an obvious removal effect for acenaphthylene, biphenyl, fluorene, phenanthreneand anthracene, and the removal rate for acenaphthylene and biphenyl can reach 100%.

Description

【Technical field】 [0001] The invention relates to the technical field of strains, in particular to a moderately halophilic polycyclic aromatic hydrocarbon degrading bacterium and its application. 【Background technique】 [0002] Polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons, PAHs) refer to hydrocarbons composed of two or more benzene rings arranged in a linear, angular, or clustered form, which are mainly derived from the incomplete combustion of fossil fuels and the production of petroleum products. Natural processes such as forest fires and volcanic eruptions also emit PAHs, and they can gradually accumulate in the environment. Most PAHs have conjugated double bond π electrons, which can absorb ultraviolet light and emit fluorescence. With the role of environmental hormones. Therefore, the distribution of PAHs in the environment and their potential threats to human health have attracted great attention from all over the world. Many countries list a r...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10C12N1/205C12R2001/01
Inventor 崔长征陈欣石杰林匡飞刘勇弟
Owner EAST CHINA UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap