Method of increasing lincomycin output by modifying gene of streptomyces lincolnensis SLCG_2919

Abstract

The invention discloses a method of increasing the lincomycin output by modifying the gene of streptomyces lincolnensis SLCG_2919. According to the method, a TetR family transcription regulation geneSLCG_2919 is deleted from streptomyces lincolnensis by a gene engineering process to obtain an engineering strain with a high yield of lincomycin; the obtained strain can produce lincomycin through fermentation, the output is largely increased, and novel technical support is provided for increasing the lincomycin output in industrial production.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a method for improving the yield of lincomycin by transforming the gene of Streptomyces lincoeum SLCG_2919. Background technique [0002] Streptomyces can produce abundant secondary metabolites, and these secondary metabolites and their derivatives are widely used, such as antibiotics, anticancer drugs, immunosuppressants, etc. More than half of the antibiotics and anticancer drugs currently used in medicine are produced by Streptomyces. Whole-genome sequencing studies have found that Streptomyces contains multiple biosynthetic gene clusters, and these gene clusters are either silent or have weak biosynthetic ability, which need to be screened to obtain high-yielding strains for industrial production. In the past, industrial production strains were mainly obtained by physical or chemical mutagenesis. Traditional mutagenesis techniques are not only time-consuming, bu...

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Problems solved by technology

Traditional mutagenesis techniques are not only time-consuming, but also highly random, and cannot provide theoretical guidance for breeding

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