Application of mycophenolic acid in inhibiting Peronophythora litchii

A technology of Phytophthora lychee downy mildew and mycophenolic acid is applied in the application, chemical for biological control, animal repellent, etc. Application prospect, environment friendly, low toxicity effect

Active Publication Date: 2018-04-13
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complex operation of agricultural control and slow effect, most of the production areas adopt chemical control with high control effect and faster effect. However, some chemical pesticides are highly toxic and harmful to the environment. Chemical Inhibitors of Phytophthora

Method used

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  • Application of mycophenolic acid in inhibiting Peronophythora litchii
  • Application of mycophenolic acid in inhibiting Peronophythora litchii
  • Application of mycophenolic acid in inhibiting Peronophythora litchii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Mycophenolic Acid Inhibits the Activity Test of Lychee Downy Phytophthora Mycelia Growth

[0034] 1. Culture medium: Carrot juice agar medium CA and potato dextrose agar medium PDA are used for cultivating Phytophthora lychee.

[0035] 2. Determination of the inhibitory effect of different concentrations of the compound mycophenolic acid on the mycelial growth of pathogenic bacteria by the method of mycelial growth rate.

[0036] Specific operation:

[0037] (1) Dissolve the pure product of the compound mycophenolic acid in DMSO, and prepare solutions with different gradient concentrations as the test solution.

[0038] (2) Add a certain amount of the prepared test solution to the CA medium whose temperature drops to 50ºC after melting and mix well, so that the final concentrations of the compounds in the medium are 0.1, 0.5, 1, 3, 5, 10, 20, 30, 100, 200 μg / mL, each concentration was treated with three replicates, and the same volume of blank solvent was ad...

Embodiment 2

[0043] Example 2 The activity test of mycophenolic acid inhibiting sporangium germination of Lychee downy mildew

[0044] 1. Preparation of pathogenic bacteria sporangia suspension:

[0045] Cultivate Phytophthora lychee in PDA medium for 5 days, add 5 mL of sterile water to the plate for washing and shaking, and prepare sporangia suspension mother solution. Add sterilized water to the sporangia suspension mother liquor, obtain the concentration of the sporangia of Lychee peronospora after dilution is 10 5 individual / mL.

[0046] 2. The spore germination method was used to determine the inhibitory effect of different concentrations of the compound mycophenolic acid on the sporangium germination of P. litchi peronospora.

[0047] Specific operation:

[0048] (1) Dissolve the compounds in DMSO, and prepare test solutions with concentrations of 1 mg / mL and 100 μg / mL, respectively.

[0049] (2) Accurately add a certain volume of test solution to the diluted sporangia suspensio...

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Abstract

The invention discloses application of mycophenolic acid in inhibiting Peronophythora litchii. It is concluded through reaches for the first time that mycophenolic acid has an important use in preventing and treating Peronophythora litchii, particularly inhibiting growth of mycelia of Peronophythora litchii and emergence of sporangium of Peronophythora litchii, thereby effectively inhibiting occurrence of Peronophythora litchii. In addition, mycophenolic acid can inhibit the growth of pathogens but can never completely kill them, and it is indicated that mycophenolic acid has low toxicity andis more environmentally friendly. Therefore, the mycophenolic acid has a promising application prospect in preventing and treating Peronophythora litchii.

Description

technical field [0001] The invention relates to the technical field of plant diseases, in particular to the application of mycophenolic acid in inhibiting litchi frost blight. Background technique [0002] Litchi is the best fruit in Lingnan of my country. It is famous both at home and abroad for its beautiful color, aroma and taste, and is known as the "King of Fruits". In addition to being eaten fresh and dried, lychee pulp can also be poured, soaked, brewed, and made into other processed products. It is an important raw material for the development of the food industry. Its industry has played a role in promoting the economic and trade development in southern my country. has high economic value. In production, due to the infection of some diseases, the quality and yield of litchi are seriously affected, among which frost blight of litchi is the most prominent. Lychee frost blight caused by Lychee downy mildew ( Peronophythora litchii ) caused by infection. The occurren...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/08A01P3/00
CPCA01N43/08
Inventor 贺飞李晓霞姜子德张智涵钟月习平根
Owner SOUTH CHINA AGRI UNIV
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