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Planting method capable of removing shattering grain seedlings or weeds in rice field or seed field

A planting method and shattering technology, which are applied in the fields of botanical equipment and methods, herbicides and algicides, and chemicals for biological control, etc., can solve the problems affecting the purity of hybrid seeds, the impact of rice yield and quality, etc.

Active Publication Date: 2018-04-20
HUNAN HYBRID RICE RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a planting method that can remove shattered seedlings and weeds in rice fields or seed production fields by using the existing herbicide resistance gene resources and the latest technology of gene fixed-point editing to solve the problem of rice cultivation. In the process of model development, the impact of field shattered seedlings and weeds on the overall yield and quality of rice, and the practical problems that shattered seedlings (miscellaneous grains) seedlings in hybrid rice production fields affect the purity of hybrid seeds

Method used

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  • Planting method capable of removing shattering grain seedlings or weeds in rice field or seed field
  • Planting method capable of removing shattering grain seedlings or weeds in rice field or seed field
  • Planting method capable of removing shattering grain seedlings or weeds in rice field or seed field

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Effect test

Embodiment 1

[0033] A kind of planting method that the present invention can remove rice seedlings and weeds, such as Figure 4 shown, including the following steps:

[0034] Through cross breeding and molecular marker-assisted selection, Huazhan with imidazolinone herbicide resistance was obtained, and the Huazhan contained a homozygous dominant genetic resistance gene. The Huazhan and Longke 638s were aligned by CRISPR / Cas9 technology, respectively. bel The genes were subjected to site-directed mutation, so that they all obtained the homozygous recessive herbicide-sensitive genes of bendazone and sulfonylurea, and the modified Huazhan and Longke 638s hybrid seeds were used to obtain imidazolinone-resistant herbicides. Longliang Youhuazhan, who is also sensitive to Bentazone.

[0035] In the first batch, Longliang Youhuazhan (both resistant to imidazolinone herbicides and sensitive to bentazon) after direct seeding transformation was sprayed with Dou Xerox (Shandong Xianda Agrochemical...

Embodiment 2

[0040] A kind of planting method that the present invention can remove rice seedlings and weeds, such as Figure 5 shown, including the following steps:

[0041] glyphosate resistance gene CP4 R9311 was transformed into R9311 by Agrobacterium-mediated method, and homozygous stable glyphosate-resistant R9311 was obtained by self-breeding. The R9311 and Y58s were aligned by CRISPR / Cas9 technology bel The genes were subjected to site-directed mutation, so that they all obtained the homozygous recessive bentazone and sulfonylurea herbicide-sensitive genes, and the modified R9311 and Y58s were used to produce hybrid seeds that were both glyphosate-resistant and bentazone-sensitive The Y You No. 1.

[0042] In the first batch, Yyou No. 1 (both resistant to glyphosate and sensitive to bendazone) after direct seeding transformation, was sprayed with Roundup at a volume concentration of 0.66% (Monsanto Company of the United States, 41% glyphosate isocyanate) at the seedling stage....

Embodiment 3

[0047] A kind of planting method that the present invention can remove rice seedlings and weeds, such as Figure 6 shown, including the following steps:

[0048] glufosinate resistance gene bar The restorer line Huazhanzhong was transformed into the restorer line Huazhanzhong by Agrobacterium-mediated method, and homozygous and stable Huazhan with glufosinate-ammonium resistance was obtained through self-breeding. The Huazhan and Jing 4155s were subjected to site-directed mutations of their Bel genes by CRISPR / Cas9 technology, so that they both obtained homozygous recessive bentazone and sulfonylurea herbicide-sensitive genes, and the transformed Huazhan Hybrid seed production with Jing 4155s yielded Jingliangyouhuazhan which is resistant to glufosinate-ammonium and sensitive to bentazone.

[0049] In the first batch, Jingliang Youhuazhan (both resistant to glufosinate and sensitive to bentazon) after direct seeding transformation was sprayed with 0.83% Baoshida (Bayer Germ...

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Abstract

The invention discloses a planting method capable of removing shattering grain seedlings or weeds in a rice field or a seed field. The problems that the whole yield and quality of rice are affected byshattering grain seedlings or weeds in a rice cultivation mode development process and the purity of hybrid seeds is affected by cut-crossing of shattering grains (hybrid grains) in a hybrid rice seed field are effectively solved by adopting a planting way of planting a rice variety or strain not only containing X herbicide resistant gene for dominant heredity, but also containing a homozygous bentazon susceptible gene for recessive heredity in a current stubble and planting a bentazon insusceptible rice variety or strain in the next stubble by using the existing latest herbicide resistant gene resource and gene fixed-point editing technology and selecting a corresponding type of herbicide to be sprayed.

Description

technical field [0001] The invention belongs to the technical field of rice planting, and in particular relates to a planting method capable of removing grain seedlings and weeds in paddy fields or seed production fields. Background technique [0002] With the development of my country's society and economy, light and simple and mechanized cultivation will become the main technical measures for rice planting. This change in planting methods has made the problem of seedlings and weeds in the field shattered grains (dropped grains from the previous rice crop) increasingly prominent. In addition, the cluster powder of shattered grains (miscellaneous grains) seedlings in the hybrid rice production field affects the purity of hybrid seeds. Problems persist too. The degree of agricultural mechanization in developed countries in Europe and the United States is very high, so crop herbicide resistance is very common. There are a series of genetic resources for crop herbicide resista...

Claims

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Application Information

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IPC IPC(8): A01G22/22A01G13/00A01N43/88A01N43/50A01N57/20A01P13/00
CPCA01G13/00A01N43/50A01N43/88A01N57/20
Inventor 赵炳然韶也毛毕刚彭彦胡远益唐丽李曜魁张丹袁志成罗武中袁隆平
Owner HUNAN HYBRID RICE RES CENT
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