Cultivation method for rapid propagation of Acer pentaphyllum twigs

A cultivation method, the technology of Acer microphylla, which is applied in the field of rapid propagation of Acer pentaphyllum branches, can solve the problems of low germination rate, slow propagation speed, and difficulty in seed collection, so as to facilitate the acquisition of materials, increase the survival rate and yield, and reduce the The effect of matching costs

Inactive Publication Date: 2018-04-24
MIANYANG TEACHERS COLLEGE
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  • Abstract
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Problems solved by technology

[0003] In summary, the existing problems in the prior art are: the current artificial propagation technology of Acer pentaphyllum onl

Method used

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  • Cultivation method for rapid propagation of Acer pentaphyllum twigs
  • Cultivation method for rapid propagation of Acer pentaphyllum twigs
  • Cultivation method for rapid propagation of Acer pentaphyllum twigs

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Example Embodiment

[0037] Example 1:

[0038] The method for cultivating the rapid propagation of Acer quinquefolia branches provided in the embodiment of the present invention includes the following steps:

[0039] (1) Collect five-leaf acer sprouts with shoots of 4-6cm in length and less than 3 years old as explant materials;

[0040] (2) Disinfection and sterilization

[0041] Disinfection and sterilization of shoots with buds: cut off the leaves of the shoots with buds, cut into shoots with buds, and sterilize the shoots with buds. The specific disinfection process is: disinfect with 75% ethanol for 10-40s, and then use sterile water Rinse 2 to 5 times, then use 0.01% HgCl 2 The solution is soaked for 3-6min; treated with 75% ethanol and treated with different concentrations (5%, 10% and 20%) of sodium hypochlorite solution for different time (5, 15, 30min), washed with sterile water 8 times and inoculated. Record the seed contamination rate and survival rate. Soak up the water, and the results of...

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Abstract

The invention belongs to the technical field of Acer pentaphyllum cultivation, and discloses a cultivation method for rapid propagation of Acer pentaphyllum twigs. Budding twigs are adopted to breed Acer pentaphyllum seedlings, the budding twigs have a plurality of budding stem segments, so that materials are easy to obtained; after the budding twigs are sterilized, germination of clustered shootscan be induced, less materials are needed, and inducements are not limited by small number of amount and seeds of existing Acer pentaphyllum theoretically so that germination amount and germination efficiency of the clustered shoots and axillary buds of the budding stem segments can reach the maximum in a short time. According to the cultivation method, under the premise of ensuring maximum proliferation efficiency, continuous germination of the clustered shoots and the axillary buds of the budding stem segments in the process of proliferative cultivation is promoted to improve survival rateand yield of the Acer pentaphyllum seedlings; adding new ingredients in a strong seedling medium is not needed, so that proportioning costs of the medium are greatly reduced, and by adopting the cultivation method to conduct seedling hardening, seedling-hardening survival rate of the Acer pentaphyllum seedlings can reach more than 80%.

Description

Technical field [0001] The invention belongs to the technical field of Acer quinquefolia cultivation, and particularly relates to a method for cultivating the branches of Acer quinquefolia for rapid propagation. Background technique [0002] Acer quinquefolia is an endemic species in Sichuan, China, which grows in river valleys at an altitude of 2200-3000 meters. Acer quinquefolia was first discovered by J.F.Rock in Muli in 1929 and named by Professor Diels of Germany in 1931. Deciduous trees, up to 10 meters high. The bark is dark brown or gray-brown, often split into irregular flakes and fall off. The branchlets are cylindrical and glabrous; the original branches are purple, and the perennial branches are purple-brown with oval lenticels. Winter buds are conical, ovate scales, sharp-pointed apex, purple, and arranged in complex tiles. Palmate compound leaf, with leaflets 4-7, usually 5; leaflets papery, narrow lanceolate, 5-9 cm long, 1.4-1.7 cm wide, apex acute, base cunea...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 胡进耀蒋炜王虹别鹏飞向莉马月琴
Owner MIANYANG TEACHERS COLLEGE
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