Cultivation method for rapid propagation of Acer pentaphyllum twigs
A cultivation method, the technology of Acer microphylla, which is applied in the field of rapid propagation of Acer pentaphyllum branches, can solve the problems of low germination rate, slow propagation speed, and difficulty in seed collection, so as to facilitate the acquisition of materials, increase the survival rate and yield, and reduce the The effect of matching costs
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[0038] The method for cultivating the rapid propagation of Acer quinquefolia branches provided in the embodiment of the present invention includes the following steps:
[0039] (1) Collect five-leaf acer sprouts with shoots of 4-6cm in length and less than 3 years old as explant materials;
[0040] (2) Disinfection and sterilization
[0041] Disinfection and sterilization of shoots with buds: cut off the leaves of the shoots with buds, cut into shoots with buds, and sterilize the shoots with buds. The specific disinfection process is: disinfect with 75% ethanol for 10-40s, and then use sterile water Rinse 2 to 5 times, then use 0.01% HgCl 2 The solution is soaked for 3-6min; treated with 75% ethanol and treated with different concentrations (5%, 10% and 20%) of sodium hypochlorite solution for different time (5, 15, 30min), washed with sterile water 8 times and inoculated. Record the seed contamination rate and survival rate. Soak up the water, and the results of the disinfection t...
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