A microfluidic chip of a bionic multi-energy biological interface system and its preparation method

A microfluidic chip and interface technology, applied in biochemical equipment and methods, methods of supporting/immobilizing microorganisms, methods of sterilization, etc., can solve problems such as limitations and difficulties in implementing Transwell experiments for microfluidic chips.

Active Publication Date: 2021-10-15
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult for the microfluidic chip to realize the function of the Transwell experiment, which is greatly limited in the research of drug ADME and cell migration.

Method used

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  • A microfluidic chip of a bionic multi-energy biological interface system and its preparation method
  • A microfluidic chip of a bionic multi-energy biological interface system and its preparation method
  • A microfluidic chip of a bionic multi-energy biological interface system and its preparation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] chip indication

[0029] A microfluidic chip of a biomimetic multifunctional biological interface system such as figure 2 As shown, the microfluidic chip is mainly composed of a top chip, a porous filter membrane, and a bottom chip. Adhesive sealing on the upper surface;

[0030] The top chip is formed by connecting the top chip main channel 1 and the top chip main channel entrance 10;

[0031] The bottom chip is composed of the left main channel 3, the right main channel 4, the collagen channel 5, the collagen channel entrance 6, the bottom chip left main channel entrance 8 and the bottom chip right main channel entrance 9, and the collagen channel 5 is connected to the left The main channel 3 on the left, and the main channel 4 on the right;

[0032] The main channel 1 of the top chip is connected to the left main channel 3 of the bottom chip through the porous filter membrane 2; the left main channel 3 of the bottom chip is connected to the main channel 1 of the ...

Embodiment 2

[0037] chip transparent effect

[0038] Make a microfluidic chip, perfuse collagen in collagen channel 5, and after curing, perfuse PBS buffer in each channel, add red dye to channel 1 of the top chip, and take a picture, as image 3 As shown in a; the photo was taken after adding the dye and standing for 2 hours, the red dye has penetrated into the main channel 3 on the left side of the bottom chip, as shown image 3 As shown in b; add the dye and let it stand for 4 hours to take pictures, the red dye has filled the main channel 3 on the left side of the bottom chip, and penetrated into the main channel 4 on the right side of the bottom chip in large quantities, as shown image 3as shown in c. The system has good permeability and has the function of forming a good biological interface.

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Abstract

The invention provides a microfluidic chip of a bionic multi-energy biological interface system and a preparation method thereof. The microfluidic chip is mainly composed of a top chip, a porous filter membrane and a bottom chip. On the surface, the lower surface of the porous filter membrane sealed with the top chip is bonded and sealed with the upper surface of the bottom chip through PDMS; the microfluidic chip prepared by this preparation method has a multi-interface structure, which can simulate the construction of various biological interfaces, and can be applied For cell co-culture, cell barrier construction, ADME research of drugs, cell migration and other research.

Description

technical field [0001] The invention relates to the field of microfluidic chip preparation, in particular to a microfluidic chip of a bionic multi-energy biological interface system and a preparation method thereof. Background technique [0002] Animal experiments occupy an extremely important position in modern medicine and biology, but funding and animal ethics have also become unavoidable issues. Combining microfluidic technology and bioscience technology, an "organ chip" has been created, which can replicate the functions of human organs with microchips, making medical experiments easier. Constructing organ-on-a-chip, especially the integration of multiple organ-on-chips to reproduce the complex in vivo environment of the human body and the interaction between organs is the general trend of future science, which requires the construction of multi-level biological interfaces to achieve. [0003] Transwell experimental technology, the traditional method is to put the Tran...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/12C12M1/00B01L3/00
CPCB01L3/5027B01L3/502707B01L3/502761B01L2200/027B01L2200/0631B01L2200/10B01L2300/0861B01L2300/0887C12M23/16C12M29/04
Inventor 秦建华李中玉许慧郭雅琼
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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