Method for detecting environmental behaviors and biological effects of nanoparticle pollutants

A nanoparticle and biological effect technology, applied in the direction of nanoparticle analysis, particle and sedimentation analysis, measuring devices, etc., can solve the problems of environmental behavior and biological effect detection without nanoparticle pollutants, achieve good guiding significance, intuitive observation, Simple operation effect

Pending Publication Date: 2021-11-16
山东大学深圳研究院
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Problems solved by technology

[0007] So far, there are no research reports using micromodels and phospholipid-layered vesicles to detect the environmental behavior and biological effects of nanoparticle pollutants

Method used

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  • Method for detecting environmental behaviors and biological effects of nanoparticle pollutants
  • Method for detecting environmental behaviors and biological effects of nanoparticle pollutants
  • Method for detecting environmental behaviors and biological effects of nanoparticle pollutants

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Embodiment Construction

[0032] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the drawings in the embodiments of the present invention.

[0033] The invention provides a method for detecting the environmental behavior and biological effects of nanoparticle pollutants, comprising the following steps:

[0034] In the first step, the micromodels and fluorochrome-stained vesicle suspensions required for the assay are prepared.

[0035] Design and manufacture micro-models to simulate the water environment and soil environment according to the needs. The size, internal structure, material, and production methods of the micro-models can be customized according to the needs of the experiment. The micromodel examples mentioned below are micromodels prepared by soft photolithography using polydimethylsiloxane (PDMS) as raw material used in our experiments. PDMS has a similar potential to silica, and the surface is easy t...

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Abstract

The invention discloses a method for detecting environmental behaviors and biological effects of nanoparticle pollutants. The method comprises the following steps: preparing a micro-model and a vesicle suspension dyed by a fluorescent dye; injecting the vesicle suspension into the micro-model from a sample inlet; introducing a background solution into the micro-model from the sample inlet, and flushing unloaded vesicles; and introducing a suspension sample of nanoparticle pollutants into the micro-model from the sample inlet, connecting a microscope with a CCD, taking pictures at fixed time, observing the fluorescence intensity in the micro-model and the distribution change of the nanoparticle pollutants, collecting effluent from a sample outlet, measuring the fluorescence intensity at fixed time by using a fluorospectro photometer, and judging whether the nanoparticle pollutants interact with the phospholipid layer or not. According to the method disclosed by the invention, environmental behaviors such as flowing, migration and the like of the nanoparticle pollutants in an environmental medium can be monitored when the biological interface exists; and the interaction between the nanoparticle pollutants and cell membranes can be detected, and the biological effect of the nanoparticle pollutants can be evaluated.

Description

technical field [0001] The invention relates to the technical field of nanoparticle pollutant detection, in particular to a method for detecting the environmental behavior and biological effects of nanoparticle pollutants. Background technique [0002] With the continuous progress and development of nanotechnology, various new nanomaterials emerge in an endless stream, have entered people's production and life, and are widely used in military, aerospace, biology, medicine, environment and other fields. Therefore, it is of great significance to explore the environmental behavior and biological effects of various nanomaterials. [0003] The existing environmental quality monitoring is to take samples at sampling points, and then bring the samples to be tested back to the laboratory, and determine the types, concentrations and trends of pollutants in the samples to be tested through a series of detection methods, so as to evaluate the environmental pollution status. However, f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/00G01N21/64
CPCG01N15/00G01N21/64G01N21/6458G01N21/643G01N21/6428G01N2015/0038G01N2015/0053G01N2021/6417G01N2021/6439
Inventor 姜威宋健张博雯
Owner 山东大学深圳研究院
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