Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Micro-fluid driving method applied to organ chip

A technology of organ chips and driving methods, applied in biochemical equipment and methods, biochemical instruments, enzymology/microbiology devices, etc., can solve the problems of rare organ chips, and achieve the promotion of drug utilization, better cell function, and longer residence time Effect

Inactive Publication Date: 2018-04-24
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, it is relatively rare to use organ chips with special fluid drive technology for relevant research and analysis, and it has great application prospects in biological research and pharmaceutical research and development.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Micro-fluid driving method applied to organ chip
  • Micro-fluid driving method applied to organ chip
  • Micro-fluid driving method applied to organ chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] microfluidic actuation method

[0022] Fabricate a single-channel microfluidic chip, the channel structure is long and straight, and the system is 50 μL. The channel is filled with water and perfused with red dye to compare the three perfusion methods. Set the parameters of the control pump to move forward continuously, and the flow rate is 1 μL / min; set the parameters of the control pump to move forward continuously, and the flow rate is 0.5 μL / min; Seconds, flow rate -1 μL / min, time 3s, pause 20 μs, cycle number 10000 times, the fluid flow rate is 0.5 μL / min. Record the time for the red dye to pass through the channel. Such as figure 1 As shown, the peristaltic flow has the shear force and fluid interaction of high flow rate, but also has the residence time and velocity of low flow rate. Its mixing effect is also better than the other two effects, such as figure 2 shown. Example 2

Embodiment 2

[0023] Penetration test

[0024] Make Transwell two-layer microfluidic chip, such as image 3 a, b, c, d, e show. The chip is composed of an upper chip, a lower chip and a porous filter membrane. The upper chip channel 13 in the upper chip is connected to the upper chip channel inlet 16, and the lower chip channel 15 is connected to the lower chip channel inlet 17 in the lower chip. The upper chip channel 13 and the lower chip channel 15 are connected through a porous filter membrane.

[0025] Fill the upper chip channel with water, use 10 mg / mL sodium fluorescein dye for perfusion, and replace with water after 25 μL. Three perfusion methods were used for the upper chip. Set the parameters of the control pump to move forward continuously, and the flow rate is 1 μL / min; set the parameters of the control pump to move forward continuously, and the flow rate is 0.5 μL / min; set the parameters of the control pump to move forward continuously, and the flow rate is 0.5 μL / min; , t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a micro-fluid driving method applied to an organ chip, and belongs to the technical field of micro-fluidics. According to the method, motion modes, namely alternated moving forwards and backwards, of fluid on the organ chip are controlled by virtue of a control pump; a peristaltic flow is formed in a micro-channel of the organ chip; and through the peristaltic flow, bidirectional shear force of the fluid is presented, the retention duration of the fluid in the micro-channel is prolonged and a fluid contact area is increased, so that a better fluid mixing effect is achieved. The driving method is stable in effect; fluid, in a peristaltic mode, in an organ can be simulated, so that micro-fluid functions are enriched; construction of the organ chip and a micro-reactor in an in-vitro simulation environment can be achieved; and the method has an important significance for application to drug evaluation and the like.

Description

technical field [0001] The invention relates to the field of a construction method and an application method of an organ chip, in particular to a microfluidic driving method on an organ chip. Background technique [0002] Animal experiments occupy an extremely important position in modern medicine and biology, but funding and animal ethics have also become unavoidable issues. Combining microfluidic technology and bioscience technology, an "organ chip" has been created, which can replicate the functions of human organs with microchips, making medical experiments easier. [0003] Microfluidic chip laboratory, also known as chip laboratory or microfluidic chip, refers to the basic operation units involved in the fields of biology and chemistry, such as sample preparation, reaction, separation, detection, cell culture, sorting, lysis, etc. Integrated or basically integrated on a chip of a few square centimeters (or even smaller), a network of microchannels is formed, and a cont...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/36
CPCC12M23/16C12M41/00
Inventor 秦建华李中玉郭雅琼
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com