Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for constructing a bionic intestinal model based on microfluidic technology

A construction method and technology of biomimetic intestines, applied in biochemical equipment and methods, artificial cell constructs, biochemical instruments, etc., to achieve the effects of promoting cell growth, improving cell function, and increasing residence time

Active Publication Date: 2021-10-15
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is no related research on the use of peristaltic flow to construct intestinal models. This bionic intestinal chip has great application prospects in biological research and pharmaceutical research and development.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for constructing a bionic intestinal model based on microfluidic technology
  • A method for constructing a bionic intestinal model based on microfluidic technology
  • A method for constructing a bionic intestinal model based on microfluidic technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Construction and Characterization of Biomimetic Intestine Model

[0030] Design and manufacture chips, collagen processing chip channel; intestinal cells adjusted to 1 ~ 5 × 10 6 The cells / mL cell suspension was added to the upper chip channel, and the cells were attached to the porous filter membrane interface to grow. The chip was moved and placed in a 37°C incubator to continue culturing. After 12 hours, the microfluidic driving method was used for continuous perfusion. Set the parameters of the control pump as 9s forward and 3s backward alternately, the flow rate is 1 μL / min, the actual effect is 0.5 μL / min, and the perfusion culture is used as the PF group. The static condition culture simulates Transwell as the control group Staic. Functional characterization of the intestinal model was performed for each group at 5 days of culture.

[0031] The surface structure of intestinal cells in each group was characterized by scanning electron microscopy, and the results...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A method for constructing a bionic intestinal model based on microfluidic technology, which is applied to the construction of an in vitro intestinal model. The microfluidic chip prepared by this preparation method is mainly composed of an upper chip, a porous filter membrane, and a lower chip. A peristaltic flow is formed in the body, and this peristaltic flow is used to simulate the actual fluid situation in the intestinal tract in the body, which has a better effect on promoting intestinal function. It is of great significance for the realization of intestinal chip in vitro simulation environment and the application of drug evaluation.

Description

technical field [0001] The invention relates to a construction method of a bionic intestinal model, in particular to a construction method of a bionic intestinal model based on microfluidic technology. Background technique [0002] Animal experiments occupy an extremely important position in modern medicine and biology, but funding and animal ethics have also become unavoidable issues. Combining microfluidic technology and bioscience technology, an "organ chip" has been created, which can replicate the functions of human organs with microchips, making medical experiments easier. [0003] Oral administration, as a traditional way of administration, is widely accepted by people because of its relative convenience and safety. The absorption process is one of the key factors determining the bioavailability of orally administered drugs. The small intestine is the main site of oral drug absorption, so at present, various in vitro and in vivo intestinal absorption models are main...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12N5/071
CPCC12M23/16C12N5/0679
Inventor 秦建华李中玉
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com