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Glioma diagnostic marker circ9:33948374|33948587 and application

A diagnostic marker and technology for glioma, which is applied in biochemical equipment and methods, microbial measurement/testing, DNA/RNA fragments, etc., can solve problems such as unsatisfactory curative effects, improve clinical treatment effects, and diagnose The effect of convenience

Active Publication Date: 2018-05-04
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the current diagnosis and treatment technology has made great progress, the curative effect is still not satisfactory

Method used

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  • Glioma diagnostic marker circ9:33948374|33948587 and application
  • Glioma diagnostic marker circ9:33948374|33948587 and application
  • Glioma diagnostic marker circ9:33948374|33948587 and application

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Experimental program
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Embodiment Construction

[0013] 1. Research object

[0014] The case group consisted of 30 serum samples of glioma collected in Hunan Cancer Hospital from January 2016 to June 2017. The control group consisted of 12 healthy individuals undergoing community disease screening during the same period, and frequency matching was carried out with the cases according to gender and age (±5 years old). The samples used for the research were collected at the same period, and the conditions of sampling, packaging and storage were the same.

[0015] 2. Research Methods

[0016] (1) Preparation of serum exosomes: Take 200 μl of serum and centrifuge at 2000 g for 30 minutes at room temperature, use a micropipette to extract the supernatant into a new 600 μl centrifuge tube, add 40 μl of exosome extraction reagent (Total Exosome Isolation Reagent (from serum ), Cat. No. 4478360, Invitrogen) gently shake up and down, and incubate at 4°C for 45 minutes. After incubation, centrifuge at 10,000 g for 10 minutes at roo...

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Abstract

The invention belongs to the technical field of biology and discloses a glioma diagnostic marker circ9:33948374|33948587 and application. The content of the circ9:33948374|33948587 in serum exosome ofa glioma patient is analyzed by a fluorescent quantitative PCR (Polymerase Chain Reaction) technology; compared with a normal control group, the expression level (p is equal to 0.0007) of the circ9:33948374|33948587 in the serum exosome of the glioma patient is obviously reduced; ROC curve analysis shows that the glioma diagnostic marker has higher diagnostic value for glioma; AUC is 0.962, p isequal to 0.007, and sensitivity and specificity are 100 percent and 92.30 percent respectively.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a serum exosome circRNA marker for glioma diagnosis, and the application of a reagent for detecting the marker for preparing glioma diagnostic preparations, as well as a kit. Background technique [0002] Glioma is one of the more common malignant tumors of the central nervous system. According to WHO, it can be divided into four grades, of which grades 1-2 are low-grade gliomas with good prognosis, and grades 3-4 are high-grade gliomas. The prognosis of patients is poor, and the postoperative survival time does not exceed five years. Although the current diagnosis and treatment technology has made great progress, the curative effect is still not satisfactory. Therefore, it is an urgent research task in the field of neuroscience to find glioma diagnostic markers to diagnose and analyze patients, to diagnose the disease as soon as possible, and to choose a reasonable follow-up treatmen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/113
CPCC12N15/113C12N2310/532C12Q1/6886C12Q2600/158C12Q2600/178
Inventor 李沛瑶武明花刘涛张严余志斌刘洋赵春花
Owner XIANGYA HOSPITAL CENT SOUTH UNIV