Lactobacillus reuteri having probiotic effect and resisting ETEC (Enterotoxigenic Escherichia Coli) and application

A technology of Lactobacillus reuteri and Escherichia coli, which is applied in the field of microorganisms, can solve problems such as ecosystem pollution, bacterial drug resistance, and aquaculture hazards, and achieve the effect of reducing the load rate

Inactive Publication Date: 2018-05-08
LONGDA FOODSTUFF GRP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these drugs can reduce the diarrhea rate of piglets, the excessive use of antibiotics can cause bacteria to develop drug resistan

Method used

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  • Lactobacillus reuteri having probiotic effect and resisting ETEC (Enterotoxigenic Escherichia Coli) and application
  • Lactobacillus reuteri having probiotic effect and resisting ETEC (Enterotoxigenic Escherichia Coli) and application
  • Lactobacillus reuteri having probiotic effect and resisting ETEC (Enterotoxigenic Escherichia Coli) and application

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Experimental program
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Effect test

Embodiment 1

[0040] Embodiment 1, the isolation and identification of HCM2 bacterial strain

[0041] 1. Isolation of HCM2 strain:

[0042] HCM2 was isolated from the intestine of 6-week-old piglets. The specific isolation method is as follows:

[0043] 1. Take the content of the cecum of healthy piglets and quickly transfer it to a sterile 50ml centrifuge tube, and transport it to the laboratory on ice;

[0044] 2. Take 0.1g of the content and add 1ml of normal saline, mix well, centrifuge at 1000rmp*10min, take the supernatant, dilute and coat with solid MRS medium, and incubate at 37°C for 48h;

[0045] 3. Pick a single clone on the medium and transfer it to liquid MRS medium for 24 hours, and store the strain at -80°C;

[0046] 4. Take out the bacterial cryopreservation tube from the -80°C refrigerator, and transfer 1% to MRS medium for activation;

[0047] 5. At 37°C, after 24 hours of static culture, 1% was transferred to MRS medium for reactivation;

[0048] 6. After 24 hours of ...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to lactobacillus reuteri from piglet intestines. The lactobacillus reuteri has strong stress resistance, can inhibit ETEC (Enterotoxigenic Escherichia Coli) from growing and the load rate of the ETEC in mice intestines. The invention specifically relates to lactobacillus reuteri having a probiotic effect and resisting ETEC and application thereof. The lactobacillus reuteri is preserved in the China General Microbiological Culture Collection Center, the preservation number is CGMCC 14598, and the lactobacillus reuteri can be applied to feed additives.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of Lactobacillus reuteri derived from piglet intestines, which has strong stress resistance and can inhibit the growth of enterotoxin-producing Escherichia coli and its load in the intestinal tract of mice Specifically, it is a strain of Lactobacillus reuteri that has probiotic properties and antagonizes enterotoxic Escherichia coli and its application. Background technique [0002] The use of antibiotics in animal husbandry can prevent and treat related animal diseases caused by intensive farming to a certain extent, and improve the economic benefits of the breeding industry. However, with the widespread and excessive use of antibiotics, serious problems such as drug resistance of pathogenic bacteria, disturbance of animal gastrointestinal micro-ecological system, antibiotic residues in animal products, and environmental pollution by animal excrement ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18C12R1/225
CPCA23K10/18C12N1/20C12N1/205C12R2001/225
Inventor 董恩球钟瑾张敏张欣王天威滕坤玲陶勇赵煜炜
Owner LONGDA FOODSTUFF GRP
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