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Method for separating and purifying labda diterpenoid components in tobacco

A separation and purification, reliable technology, applied in the field of separation and purification, can solve the problems of cumbersome separation process, low compound yield, and no prospect of popularization and application, and achieves mature process operation, high recovery rate, and good popularization and application value. Effect

Active Publication Date: 2018-05-11
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For the separation and extraction of these two substances, there are some reports in the prior art, but generally speaking, the existing extraction and separation process is cumbersome, the compound yield is low, and there is no prospect of popularization and application.

Method used

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  • Method for separating and purifying labda diterpenoid components in tobacco
  • Method for separating and purifying labda diterpenoid components in tobacco
  • Method for separating and purifying labda diterpenoid components in tobacco

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Embodiment

[0033] In this embodiment, the extraction and separation of the diterpenoid components of Labatin in tobacco specifically includes the following steps:

[0034] (1) Extraction of tobacco leaves: Take the fresh tobacco leaves of mature oriental tobacco produced in Hangzhou (tobacco leaves on the day of harvest) as an example, and carry out relevant extraction and separation operations.

[0035] Fresh tobacco leaves were picked, quick-frozen with liquid nitrogen, and vacuum freeze-dried at low temperature (≤40°C). After drying, they were powdered and passed through a 100-mesh sieve for later use. Take 0.8Kg of sieved tobacco leaf powder, put it in 20L dichloromethane, soak and extract at room temperature for 3 times, 24 hours each time, replace the extract after filtering, and finally combine the extract after filtering, and keep the constant temperature water bath at 40±5°C Concentrate under reduced pressure until there is no liquid under the same conditions, and finally obtain...

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Abstract

The invention belongs to the technical field of separation and purification, and particularly relates to extraction, separation and purification of diterpenoid related substances in tobacco. The method comprises the steps of tobacco leaf extraction, normal phase silica gel column chromatography separation, high-performance liquid chromatography separation and the like. Due to the fact that the labda diterpenoid components (including Z-abienol and 13(E)-labd-13-ene-8 alpha,15-diol) mainly exist in glandular hair secretions on the surface of tobacco leaves, the raw materials for extraction mustbe fresh tobacco leaves in order to ensure better extraction effect. Preliminary detection results show that the purity of the labda diterpenoid components prepared with the extraction and separationmethod is higher than 98%, and the substances can be completely used as standard substances in analysis and research of related substances. Meanwhile, due to the relatively high recovery rate of related separation and purification products and the relatively mature and simple process operation, the method has better popularization and application value, a good methodological foundation can also belaid for improvement of tobacco quality.

Description

technical field [0001] The invention belongs to the technical field of separation and purification, and in particular relates to the extraction, separation and purification of a class of diterpenoid-related substances in tobacco. Background technique [0002] Z -abienol ( Z -Abitol, molecular formula: C 20 h 34 O) and 13( E )-labd-13-ene-8α,15-diol (Libadang-13-ene-8,15-diol, molecular formula: C 20 h 36 o 2 ) is an important class of diterpenes in tobacco, the specific chemical structures are as follows: [0003] . [0004] These two substances are also important precursors of aroma and aroma components in tobacco leaves. The two are degraded during the brewing process, and the important aroma-causing substance ambergris is produced, which has a significant impact on the quality and aroma style of tobacco leaves. At the same time, the diterpenoids in tobacco leaves have obvious effects on preventing tobacco pests and diseases, and are one of the key chemical sub...

Claims

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Application Information

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IPC IPC(8): C07C29/74C07C29/76C07C35/36
CPCC07C29/74C07C29/76C07C35/36
Inventor 郑庆霞魏攀周会娜刘萍萍陈千思金立峰翟妞陈霞张慧徐国云
Owner ZHENGZHOU TOBACCO RES INST OF CNTC