Mixed freeze-dried powder of platelet-rich plasma combined with excreted factor of human mesenchymal stem cells and preparation method thereof

A technology of stem cells and secreted factors, which is applied to the mixed freeze-dried powder of platelet-rich plasma combined with secreted factors of human mesenchymal stem cells and the production field thereof, can solve the problems of limitation, cumbersome and inconvenient subsequent use, high maintenance costs and the like, To achieve the effect of reducing the occurrence of protein mutation

Inactive Publication Date: 2018-05-22
吕瑞华
View PDF6 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The clinical application of PRP and cytokines secreted by mesenchymal stem cells in medical aesthetics, anti-aging, and regeneration will be a future trend. The storage method is stored for subsequent use, resulting in the limitation of the frozen storage environment and high mai

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mixed freeze-dried powder of platelet-rich plasma combined with excreted factor of human mesenchymal stem cells and preparation method thereof
  • Mixed freeze-dried powder of platelet-rich plasma combined with excreted factor of human mesenchymal stem cells and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings and examples.

[0023] According to a specific embodiment of the present invention, the mixed freeze-dried powder of platelet-rich plasma combined with human mesenchymal stem cell-secreted factors of the present invention includes a platelet-rich plasma powder and mesenchymal stem cell-secreted factor powder. Wherein, the weight percentage of the platelet-rich plasma powder contained in the mixed freeze-dried powder is greater than the weight percentage of the mesenchymal stem cell secreted factor powder.

[0024] The platelet rich plasma (Platelet Rich Plasma, PRP) is obtained from the blood of the user who intends to use the mixed freeze-dried powder.

[0025] Since the platelet-rich plasma used is obtained from the user's own blood, it will not cause hepatitis, AIDS...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses mixed freeze-dried powders of platelet-rich plasma combined with excreted factors of human mesenchymal stem cells and a preparation method thereof. The method comprises steps:preparing platelet-rich plasma; culturing excreted factor of mesenchymal stem cells; performing freeze-drying on the platelet-rich plasma and the excreted factor of mesenchymal stem cells, and mixingthe platelet-rich plasma and the excreted factor of mesenchymal stem cells, wherein weight percentage of self platelet-rich plasma contained in the mixed freeze-dried powders is larger than that of the excreted factor of mesenchymal stem cells. Autoblood and human cells are used as materials, to improve human absorption benefit and security, and a used nutrient solution is human platelet serum butnot animal fetal calf serum, to reduce protein variation. A freeze-drying technology is used to overcome a deterioration problem caused by heat sensitivity and aging, and meanwhile ratio of the mixedfreeze-dried powders can be customized, so as to be used in different environments later.

Description

technical field [0001] The present invention relates to a freeze-dried powder and its production method, and in particular, to a mixed freeze-dried powder combined with autologous platelet-rich plasma and human mesenchymal stem cell secretion factors and its production method. Background technique [0002] Platelet rich plasma (platelet rich plasma, PRP) contains a large number of biologically active platelets. Under certain conditions, these platelets can release various cytokines stored in it to the surrounding environment through the activation process to promote blood circulation. Cell proliferation, differentiation and tissue reconstruction and repair, therefore, it is a novel and convenient technology for treatment and medical aesthetics. [0003] Autologous platelet plasma technology can not only be applied to orthopedic treatment, but also has been widely used in the treatment of chronic wounds such as diabetic foot and decubitus. At the same time, autologous platele...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K35/28A61K35/33A61K9/19A61P39/06A61K8/98A61K8/02A61Q19/08A61K35/16
CPCA61K8/022A61K8/981A61K8/982A61K8/983A61K9/0014A61K9/0019A61K9/19A61K35/16A61K35/28A61K35/33A61K2800/91A61Q19/08A61K2300/00
Inventor 吕瑞华
Owner 吕瑞华
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap