Method for detecting fixation strength of microorganism and pollutant purifying carrier at high precision

Abstract

The invention provides a method for detecting the fixation strength of a microorganism and a pollutant purifying carrier at high precision. The method specifically comprises the following steps: (1) preparing the pollutant purifying carrier loaded with the microorganism; (2) setting N experimental groups according to the sampling time; (3) washing the pollutant purifying carrier loaded with the microorganism with running water, sampling and storing a water body and the pollutant purifying carrier; (4) extracting DNAs of the pollutant purifying carrier and the microorganism of the water body, and selecting a PCR (Polymerase Chain Reaction) amplification primer; (5) preparing a standard curve sample for detecting the richness of the microorganism; (6) configuring a PCR reaction system, setting a reaction program, and conducting real-time fluorescence quantitative PCR; and (7) calculating the richness, the fixation rate and the effective fixation time of the microorganism on the water body and the pollutant purifying carrier. The method has the advantages that (1) the detection precision is high, and the error is small; (2) different microorganism groups are precisely distinguished; (3) the detection is conducted rapidly in real time; and (4) the technological means is mature, simple and convenient.

Description

technical field [0001] The invention relates to a method for high-precision detection of the fixation strength of microorganisms and pollution carriers, and belongs to the technical field of water environment restoration. Background technique [0002] In the field of water environment remediation technology, microbial remediation has the advantages of low energy consumption, no secondary pollution, high efficiency, simple operation, economical and practical, and wide application range. It is favored and has become an important way to remove pollutants in the water environment. In actual in situ remediation applications, in order to play the role of indigenous microorganisms or specific functional bacteria obtained by artificial screening, the microorganisms are usually coupled and attached to the decontamination carrier by means of natural processes, artificially applied physical or chemical methods, and the original free The microbial load may be limited to a specific surf...

AI Technical Summary

Problems solved by technology

However, due to the limited methods for detecting the fixation strength of microorganisms and decontamination carriers at present, and the accuracy of existing methods is poor, most decontamination carriers loaded with microorganisms are blindly put into in-situ application without fixation strength detection, causing Severe microbial shedding, poor repair effect, high biological risk and other consequences

In the Chinese patent document whose publication number is CN103940695A, a method for testing the microbial fixation strength of carbon fiber carrier is disclosed. The fixation strength has been quantitatively tested, but ultrasonic-oscillation is not a real condition in the actual water environment, and the weighing method has disadvantages such as low accuracy, poor timeliness, and inability to distinguish different microbial groups. It is urgent to develop a high-precision, Small error, different microbial groups can be distinguished, convenient and fast detection method for the fixation strength of microorganisms and cleaning carriers

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