SNP (Single Nucleotide Polymorphism) marker relative to soybean seed methionine content as well as interval, primer and application of SNP marker

A technology of seed methionine and methionine, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problems of undiscovered, genetic improvement molecular marker-assisted selection and breeding difficulties, etc., to improve efficiency and reduce The effect of choosing cost and speeding up the improvement process

Inactive Publication Date: 2018-05-22
JILIN ACAD OF AGRI SCI
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Problems solved by technology

However, no SNP (Single nucleotide polymorphism, single nucleotide polymorphism) molecular markers related to the methionine content traits of soybean seeds have been found, which brings difficulties to the genetic improvement of soybean seed methionine content traits and molecular marker-assisted selection breeding

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  • SNP (Single Nucleotide Polymorphism) marker relative to soybean seed methionine content as well as interval, primer and application of SNP marker
  • SNP (Single Nucleotide Polymorphism) marker relative to soybean seed methionine content as well as interval, primer and application of SNP marker
  • SNP (Single Nucleotide Polymorphism) marker relative to soybean seed methionine content as well as interval, primer and application of SNP marker

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Embodiment Construction

[0023] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, but should not be construed as a limitation of the present invention. Unless otherwise specified, the technical means used in the following examples are conventional means well known to those skilled in the art, and the materials, reagents, etc. used in the following examples, unless otherwise specified, can be obtained from commercial sources.

[0024] In the following examples, 3000 copies of soybean germplasm resources were widely collected in the north of 40°N in my country, and the content of methionine in soybean seeds was determined by high performance liquid chromatography. According to the distribution of methionine in the resources, each distribution group was sampled, and then Compare the genetic diversity of the sampling population and the total population, and determine the number of sampling samples when they are consistent. A total...

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Abstract

The invention belongs to the technical field of soybean molecular biology and particularly relates to a SNP (Single Nucleotide Polymorphism) marker relative to a soybean seed methionine content as well as an interval, a primer and application of the SNP marker. The SNP marker is positioned 3233657 locus of chromosome No.10 of soybean, and nucleotide marked by the molecule is G or A; the nucleotidecontaining the mark sequence is shown as SEQ ID NO. 1; and the marker interval is an interval from 3233657 locus to -3243657 locus of the chromosome No.10 of the soybean. The SNP marker belongs to adominant marker, is reliable, is convenient to use, provides very great convenience to soybean high methionine content strain breeding, can be applied to molecular marker-assisted selection in soybeanseed methionine content improvement as well as fine mapping and map-based cloning of genes relative to the seed methionine content and thus accelerates the improvement process of the soybean seed methionine content character.

Description

technical field [0001] The invention belongs to the technical field of soybean molecular biology, and specifically relates to a SNP marker, interval, primer and application related to the methionine content of soybean seeds. Background technique [0002] Soybean protein is one of the important sources of nutrition for humans and poultry. Its amino acid composition is similar to that of milk protein. It is a relatively complete protein, except for the lack of methionine necessary for human body. The quality of soybean protein affects the market competitiveness of soybean products, and the content of methionine is the primary limiting factor. The methionine content in soybeans is generally only 12mg / g protein, which is far from the 26mg / g recommended by the World Health Organization and the Chinese Nutrition Society, which meets the nutritional and health needs of the human body. In order to meet people's increasing nutritional needs, increasing the methionine content of cult...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 王跃强王洋邱红梅侯云龙马晓萍高淑芹陈健胡金海王丽
Owner JILIN ACAD OF AGRI SCI
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