Determination method of multiple components in longan leaves

A method of determination, the technology of longan leaves, applied in the field of medical testing, can solve the problems of waste of resources, environment, pollution, discarding or burning of leaves, etc., and achieve the effect of good repeatability, good separation effect and strong applicability

Active Publication Date: 2020-10-02
GUANGXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The resources of longan leaves in southern my country are abundant. After the longan is picked every year, the fruit growers cut off the branches of the trees, and a large number of leaves are discarded or burned, resulting in waste of resources and environmental pollution.

Method used

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  • Determination method of multiple components in longan leaves
  • Determination method of multiple components in longan leaves
  • Determination method of multiple components in longan leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0164] Take about 2g of longan leaf (origin: Wuzhou, Guangxi) coarse powder, weigh it accurately, add 10ml of methanol-hydrochloric acid (8:1) solution accurately, weigh, extract by ultrasonic for 50min, let it cool, and use methanol-hydrochloric acid (8:1) Make up the lost weight with the solution, shake well, filter, take the continued filtrate in a centrifuge tube, centrifuge at 13000r / min for 10min, take 10μl of the supernatant, inject it into a liquid chromatograph for measurement, and obtain it. The results are shown in Table 20.

Embodiment 2

[0166] Take about 3g of longan leaf (origin: Hezhou, Guangxi) coarse powder, accurately weighed, accurately add 15ml of methanol-hydrochloric acid (8:1) solution, weigh, ultrasonically extract for 60min, let it cool, and use methanol-hydrochloric acid (8:1) Make up the lost weight with the solution, shake well, filter, take the continued filtrate in a centrifuge tube, centrifuge at 13000r / min for 10min, take 10μl of the supernatant, inject it into a liquid chromatograph for measurement, and obtain it. The results are shown in Table 20.

Embodiment 3

[0168]Take about 2g of longan leaf (origin: Yulin, Guangxi) coarse powder, accurately weighed, accurately add 10ml of methanol-hydrochloric acid (8:1) solution, weigh, ultrasonically extract for 50min, let cool, and use methanol-hydrochloric acid (8:1) Make up the lost weight with the solution, shake well, filter, take the continued filtrate in a centrifuge tube, centrifuge at 13000r / min for 10min, take 10μl of the supernatant, inject it into a liquid chromatograph for measurement, and obtain it. The results are shown in Table 20.

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Abstract

The invention discloses a determination method for multi-component content in Longan leaves. In the determination method, the high-performance liquid chromatography is used to determine the content ofethyl gallate, astragalin, quercetin, luteolin and kaempferol in the longan leaves simultaneously. The method has the advantages of high efficiency, accuracy, good resolution, repeatability and stability, simple and easy operation, and can effectively control the quality of the longan leaves.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a method for determining the content of multiple components in longan leaves. Background technique [0002] Longan leaf is a traditional Chinese medicinal material with Guangxi national characteristics, which was first recorded in "Southern Yunnan Bencao Tushuo". For the famous subtropical fruit - longan (Dimocarpus longan Lour.) tree leaves or shoots. Its nature and flavor are sweet, light, and flat, and it has the functions of clearing heat, dampness and detoxification. It is mainly used to treat cold, fever, malaria, boils, eczema, etc. Longan originates in the subtropical region of southern my country and northern Vietnam, and has been cultivated in my country for more than 2,000 years. my country's longan cultivation area and output both rank first in the world. Longan leaf resources are abundant in southern my country. After the longan is picked every year, fr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 梁洁黄春燕李耀华周昱杉信晨曦杨川川
Owner GUANGXI UNIV OF CHINESE MEDICINE
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